Serum concentrations of a granulocyte-derived calcium-binding protein in cystic fibrosis patients and heterozygotes.
Serum concentrations of a granulocyte-derived calcium-binding protein in cystic fibrosis patients and heterozygotes.
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囊性纤维化患者和杂合子中粒细胞衍生的钙结合蛋白的血清浓度。
DOI：
10.1016/0009-8981(87)90382-2
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
D. Brock
中科院分区：
文献类型：
--
作者：
C. Hayward;S. Glass;V. van Heyningen;D. Brock
囊性纤维化纯合子和杂合子的血清样本含有一种被称为囊性纤维化抗原的蛋白质水平升高，这种蛋白质主要是在粒细胞中合成的。我们已经生产了抗囊性纤维化抗原的单抗，并开发了一种灵敏的双部位免疫分析方法。检测了50例囊性纤维化纯合子、34例杂合子、60例健康对照和25例疾病对照血清中的抗原水平。同时测定了另一种粒细胞来源的血清蛋白乳铁蛋白，以及急性期反应物C反应蛋白。尝试使用血清中囊性纤维化抗原的浓度来区分囊性纤维化患者和杂合子是不成功的，即使这些浓度是与乳铁蛋白或C反应蛋白的比率表达的。然而，对囊性纤维化杂合子血清样本中囊性纤维化抗原与乳铁蛋白的比率的检测表明，该抗原与囊性纤维化基因之间存在某种特定的关联。
Serum samples from cystic fibrosis homozygotes and heterozygotes contain elevated levels of a protein, known as cystic fibrosis antigen, which is synthesized primarily in granulocytes. We have produced monoclonal antibodies against cystic fibrosis antigen and developed a sensitive two-site immunoassay. Antigen levels were evaluated in serum samples from 50 cystic fibrosis homozygotes, 34 heterozygotes, 60 healthy controls and 25 disease controls. Simultaneous measurement was made of another granulocyte-derived serum protein, lactoferrin, and also of the acute-phase reactant, C-Reactive Protein. Attempts to use the concentrations of cystic fibrosis antigen in serum to distinguish cystic fibrosis patients from heterozygotes were unsuccessful, even when these concentrations were expressed as a ratio with lactoferrin or with C-Reactive Protein. However, examination of the ratio of cystic fibrosis antigen to lactoferrin in serum samples from cystic fibrosis hetrozygotes suggests that there is some specific association between this antigen and the cystic fibrosis gene.
囊性纤维化患者淋巴细胞和粒细胞中的 β 肾上腺素受体。
DOI：
10.1172/jci110934
发表时间：
1983
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Davis,PB;Dieckman,L;Boat,TF;Stern,RC;Doershuk,CF
通讯作者：
Doershuk,CF