Dosage-sensitive and complementary functions of roundabout and commissureless control axon crossing of the CNS midline
Dosage-sensitive and complementary functions of roundabout and commissureless control axon crossing of the CNS midline
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DOI：
10.1016/s0896-6273(00)80431-6
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发表时间：
1998-01-01
期刊：
影响因子：
16.2
通讯作者：
Tear, G
中科院分区：
文献类型：
--
作者：
Kidd, T;Russell, C;Tear, G
无连合和迂回导致互补突变表型，其中太少或太多轴突穿过中线。robo; robe双突变体表型与长袍单突变体表型相同，表明在长袍缺失的情况下，不再需要robe。Comm在中线细胞上表达;长袍在生长锥上以动态方式表达，并且似乎起轴突引导受体的作用。长袍是剂量敏感的。过表达的rfos也是剂量敏感的，并导致表型相同的长袍功能丧失。Comm控制长袍表达;增加Comm导致长袍蛋白的减少。Comm和长袍的水平似乎受到严格调节，以确保只有某些生长锥穿过中线，并且那些穿过中线的生长锥再也不会这样做。
commissureless and roundabout lead to complementary mutant phenotypes in which either too few or too many axons cross the midline. The robo;comm double-mutant phenotype is identical to robe alone, suggesting that in the absence of robe, comm is no longer required. Comm is expressed on midline cells; Robe is expressed in a dynamic fashion on growth cones and appears to function as an axon guidance receptor. robe function is dosage-sensitive. Overexpression of comm is also dosage-sensitive and leads to a phenotype identical to robe loss-of-function. Comm controls Robe expression; increasing Comm leads to a reduction of Robe protein. The levels of Comm and Robe appear to be tightly regulated to assure that only certain growth cones cross the midline and that those growth cones that do cross never do so again.