MOR1/MAP215 acts synergistically with katanin to control cell division and anisotropic cell elongation in Arabidopsis
MOR1/MAP215 acts synergistically with katanin to control cell division and anisotropic cell elongation in Arabidopsis
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DOI：
10.1093/plcell/koac147
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发表时间：
2022-05-17
期刊：
影响因子：
11.6
通讯作者：
Yu, Fei
中科院分区：
文献类型：
--
作者：
Chen, Yu;Liu, Xiayan;Yu, Fei
MAP215家族的微管（MT）聚合酶/成核因子和MT切断酶katanin是广泛保守的MT相关蛋白（MAPs）在植物和动物界。然而，这两种基本的map如何协调调节植物MT动态和发育仍不清楚。在这里，我们在基因筛选中发现了新的微管组织1 （MOR1）的半形等位基因，编码拟南芥MAP215的同源基因，用于对mt不稳定药物丙酰胺过度敏感的突变体。植物活体成像显示，mor1 -绿色荧光蛋白主要跟踪间期细胞皮层MT （cmt）的正端，并标记分裂细胞的前期带、梭形体和片质体MT阵列。值得注意的是，MOR1和KATANIN 1 (KTN1)（拟南芥KATANIN蛋白的p60亚基）协同作用，控制植物特异性MT阵列的适当形成，从而控制细胞分裂和各向异性细胞扩增。此外，MOR1与KTN1物理相互作用并促进KTN1介导的cMTs切断。我们的工作确定了拟南芥MOR1-KTN1相互作用是决定MT动态和植物生长发育的中心功能节点。MOR1/MAP215和KTN1是两个保守的微管（MT）相关蛋白，在细胞分裂和细胞伸长过程中相互作用并协同控制MT组织。
The MAP215 family of microtubule (MT) polymerase/nucleation factors and the MT severing enzyme katanin are widely conserved MT-associated proteins (MAPs) across the plant and animal kingdoms. However, how these two essential MAPs coordinate to regulate plant MT dynamics and development remains unknown. Here, we identified novel hypomorphic alleles of MICROTUBULE ORGANIZATION 1 (MOR1), encoding the Arabidopsis thaliana homolog of MAP215, in genetic screens for mutants oversensitive to the MT-destabilizing drug propyzamide. Live imaging in planta revealed that MOR1-green fluorescent protein predominantly tracks the plus-ends of cortical MTs (cMTs) in interphase cells and labels preprophase band, spindle and phragmoplast MT arrays in dividing cells. Remarkably, MOR1 and KATANIN 1 (KTN1), the p60 subunit of Arabidopsis katanin, act synergistically to control the proper formation of plant-specific MT arrays, and consequently, cell division and anisotropic cell expansion. Moreover, MOR1 physically interacts with KTN1 and promotes KTN1-mediated severing of cMTs. Our work establishes the Arabidopsis MOR1-KTN1 interaction as a central functional node dictating MT dynamics and plant growth and development.MOR1/MAP215 and KTN1, two conserved microtubule (MT)-associated proteins, interact and synergistically control MT organizations during cell division and cell elongation.