CHARACTERIZATION OF HOMOTYPIC AND HETEROTYPIC VP7 NEUTRALIZATION SITES OF RHESUS ROTAVIRUS
CHARACTERIZATION OF HOMOTYPIC AND HETEROTYPIC VP7 NEUTRALIZATION SITES OF RHESUS ROTAVIRUS
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DOI：
10.1016/0042-6822(88)90595-8
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发表时间：
1988-08-01
期刊：
影响因子：
3.7
通讯作者：
GREENBERG, HB
中科院分区：
文献类型：
--
作者：
MACKOW, ER;SHAW, RD;GREENBERG, HB
测定了恒河猴轮状病毒的基因9个核苷酸序列，并对14个病毒变异体的中和单抗进行了筛选。每个变异都包含一个基因9，即VP7突变，它允许病毒在抗体存在的情况下生长。在两个不同的网络化区域中发现了变异突变。A区由参与血清型特异性和血清型交叉反应中和的单抗鉴定。C区用血清型特异性网状单抗鉴定。异型中和单抗57-8选择了A区氨基酸94位突变的变异体，与血清型特异性单抗选择的氨基酸位置相同。单抗3在氨基酸99处选择了VP7突变，导致VP7蛋白的额外N-连接糖基化。尽管增加了VP7糖基化，但变异体V3对额外的VP7特异性中和单抗并不具有广泛的抵抗力。
The gene 9 nucleotide sequence was determined for rhesus rotavirus and each of 14 viral variants selected for their resistance to neutralizing monoclonal antibodies. Each variant contains a single gene 9, VP7, mutation which permits viral growth in the presence of the antibody. Variant mutations were identified in two distinct netralization regions. Region A was identified by monoclonal antibodies that are involved in both serotype-specific and serotype cross-reactive neutralization. Region C was identified by serotype-specific netralizing monoclonal antibodies. Heterotypic neutralizing monoclonal antibody 57-8 selected variants with a mutation at amino acid 94 in the A region, the same amino acid location selected by serotype-specific monoclonal antibodies. Monoclonal antibody 3 selected a VP7 mutation at amino acid 99 resulting in additional N-linked glycosylation of the VP7 protein. Despite the added VP7 glycosylation, variant v3 was not broadly resistant to additional VP7-specific neutralizing monoclonal antibodies.