Intestinal Microbiota Is Influenced by Gender and Body Mass Index.
Intestinal Microbiota Is Influenced by Gender and Body Mass Index.
复制标题
肠道菌群受性别和体重指数的影响。
DOI：
10.1371/journal.pone.0154090
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Camargo A
中科院分区：
文献类型：
--
作者：
Haro C;Rangel-Zúñiga OA;Alcalá-Díaz JF;Gómez-Delgado F;Pérez-Martínez P;Delgado-Lista J;Quintana-Navarro GM;Landa BB;Navas-Cortés JA;Tena-Sempere M;Clemente JC;López-Miranda J;Pérez-Jiménez F;Camargo A
肠道微生物区系的变化与肥胖的发生有关。然而，由于在饮食、年龄和荷尔蒙因素方面存在高度的个体间异质性，以及性别的影响在很大程度上尚未被探索，对人类的研究产生了相互矛盾的结果。在这项工作中，我们的目标是确定与肥胖相关的不同肠道微生物区系特征，作为性别和体重指数(BMI)变化的函数。通过16S测序分析了39名男性和36名绝经后女性的细菌群落结构的差异，这些人的饮食背景相似，按年龄匹配，并根据BMI进行分层。我们观察到，当体重指数为33时，男性的类杆菌属的丰度低于女性(P<；0.001，q=0.002)。事实上，在体重指数增加的男性中，该属的丰度降低(P<；0.001，Q<；0.001)。然而，在女性中，它在不同的BMI范围内保持不变。我们观察到，与女性相比，男性粪便样本中韦罗氏菌的存在(84.6%比47.2%；X2检验P=0.001，q=0.019)和甲烷短杆菌属(84.6%比47.2%；X2检验P=0.002，Q=0.026)更高。我们还观察到，无论体重指数如何，男性的比洛菲拉丰度都低于女性(P=0.002，Q=0.041)。此外，在校正了年龄和性别后，发现属水平上的66个细菌分类与BMI和血脂有关。微生物区系解释P=0.001，体重指数变异31.17%，甘油三酯变异29.04%，高密度脂蛋白33.70%，低密度脂蛋白46.86%，总胆固醇28.55%。我们的结果表明，肠道微生物区系在男性和女性之间可能不同，这些差异可能受到肥胖程度的影响。在男性和女性之间观察到的肠道微生物区系的差异可能在定义代谢性和肠炎性疾病患病率的性别差异方面起主导作用。
Intestinal microbiota changes are associated with the development of obesity. However, studies in humans have generated conflicting results due to high inter-individual heterogeneity in terms of diet, age, and hormonal factors, and the largely unexplored influence of gender. In this work, we aimed to identify differential gut microbiota signatures associated with obesity, as a function of gender and changes in body mass index (BMI). Differences in the bacterial community structure were analyzed by 16S sequencing in 39 men and 36 post-menopausal women, who had similar dietary background, matched by age and stratified according to the BMI. We observed that the abundance of the Bacteroides genus was lower in men than in women (P<0.001, Q = 0.002) when BMI was > 33. In fact, the abundance of this genus decreased in men with an increase in BMI (P<0.001, Q<0.001). However, in women, it remained unchanged within the different ranges of BMI. We observed a higher presence of Veillonella (84.6% vs. 47.2%; X2 test P = 0.001, Q = 0.019) and Methanobrevibacter genera (84.6% vs. 47.2%; X2 test P = 0.002, Q = 0.026) in fecal samples in men compared to women. We also observed that the abundance of Bilophila was lower in men compared to women regardless of BMI (P = 0.002, Q = 0.041). Additionally, after correcting for age and sex, 66 bacterial taxa at the genus level were found to be associated with BMI and plasma lipids. Microbiota explained at P = 0.001, 31.17% variation in BMI, 29.04% in triglycerides, 33.70% in high-density lipoproteins, 46.86% in low-density lipoproteins, and 28.55% in total cholesterol. Our results suggest that gut microbiota may differ between men and women, and that these differences may be influenced by the grade of obesity. The divergence in gut microbiota observed between men and women might have a dominant role in the definition of gender differences in the prevalence of metabolic and intestinal inflammatory diseases.