A REGION OF THE 5' NONCODING REGION OF FOOT-AND-MOUTH-DISEASE VIRUS-RNA DIRECTS EFFICIENT INTERNAL INITIATION OF PROTEIN-SYNTHESIS WITHIN CELLS - INVOLVEMENT WITH THE ROLE OF L-PROTEASE IN TRANSLATIONAL CONTROL
A REGION OF THE 5' NONCODING REGION OF FOOT-AND-MOUTH-DISEASE VIRUS-RNA DIRECTS EFFICIENT INTERNAL INITIATION OF PROTEIN-SYNTHESIS WITHIN CELLS - INVOLVEMENT WITH THE ROLE OF L-PROTEASE IN TRANSLATIONAL CONTROL
复制标题
DOI：
10.1128/jvi.64.11.5389-5395.1990
复制
发表时间：
1990-11-01
影响因子：
5.4
通讯作者：
BRANGWYN, JK
中科院分区：
文献类型：
--
作者：
BELSHAM, GJ;BRANGWYN, JK
已经构建了编码双顺反子mRNA的质粒，并用于鉴定口蹄疫病毒（FMDV）5“非编码区的一个区域，该区域指导细胞内蛋白质合成的有效内部起始。从435-nt区域的5“末端缺失约30个核苷酸（nt）或从3”末端缺失约50个nt完全消除了该区域的活性。FMDV L蛋白酶的表达严重抑制其他基因的表达，除非它们之前有这个元素。讨论了口蹄疫病毒介导的蛋白质合成调节。
Plasmids encoding bicistronic mRNAs have been constructed and used to identify a region from the 5'' noncoding region of foot-and-mouth disease virus (FMDV) which directs efficient internal initiation of protein synthesis within cells. The loss of about 30 nucleotides (nt) from the 5'' terminus or about 50 nt from the 3'' terminus of the 435-nt region completely abolished the activity of this region. The expression of the FMDV L protease severely inhibited the expression of other genes unless they were preceded by this element. The regulation of protein synthesis mediated by FMDV is discussed.