Free radical scavenging activity of fullerenol on the ischemia-reperfusion intestine in dogs
Free radical scavenging activity of fullerenol on the ischemia-reperfusion intestine in dogs
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DOI：
10.1007/s002689910071
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发表时间：
2000-04-01
影响因子：
2.6
通讯作者：
Chiang, LY
中科院分区：
文献类型：
--
作者：
Lai, HS;Chen, WJ;Chiang, LY
富勒烯是一种水溶性的C-60-富勒烯衍生物，已被证明在体内外具有清除自由基的能力。本研究的目的是探讨富勒烯醇在预防和治疗小肠缺血再灌注(UR)损伤时是否能清除大量诱导的自由基。夹闭雄性杂种犬肠系膜上动、静脉60min，造成IIR损伤。30只犬随机分为3组，每组10只：对照组(C)不给药，预防(P)组在缺血前30min静脉注射富勒醇(1 mg/kg)，治疗(T)组在再灌流后即刻给予相同剂量的富勒醇。这项研究是一项实验性随机试验。分别于再灌流后10、20、30、60min取肠段，再灌流60min后采血，取主要脏器标本。测定肠组织中共轭二烯(CD)、丙二醛(MDA)等脂质过氧化产物浓度及谷胱甘肽(GSH)含量。检测血清肝肾功能指标。同时对小肠和主要脏器进行组织学检查。肠组织中丙二醛和镉含量在再灌流后30min和60min显著升高。而组织中GSH含量在再灌流60min后下降。给予富勒烯醇能在预防和治疗方面减少这些变化。各组肝肾功能均在正常范围内。此外，P组和T组均未发现明显的组织病理学附加损伤。提示富勒烯醇对UR所致小肠损伤所致的自由基有较强的清除作用。
Fullerenol, a water-soluble C-60-fullerene derivative, has been demonstrated to have the capability to scavenge free radicals in vitro and in vivo. The purpose of this study was to investigate whether fullerenol can scavenge the free radicals that are massively induced during ischemia-reperfusion (UR) injury of the small intestine, either preventively or therapeutically. Clamping the superior mesenteric artery and vein for 60 minutes to induce IIR injury was performed on male mongrel dogs. Thirty dogs were divided into three groups (10 in each): The control (C) group received no medication; the preventive (P) group received fullerenol (1 mg/kg) intravenously 30 minutes before ischemia; the therapeutic (T) group received the same dose of fullerenol immediately after reperfusion. This study was an experimental randomized trial. Intestinal segments were obtained 10, 20, 30, and 60 minutes after reperfusion; and blood samples and specimens of major organs were taken 60 minutes after reperfusion. Concentrations of lipid peroxidation products, including conjugated diene (CD) and malondialdehyde (MDA), and the level of glutathione (GSH) in intestinal tissue were determined. Serum indicators of liver and renal function were measured. Histologic examination of the small intestine and major organs were also performed. A significant increase in intestinal MDA and CD contents was detected at 30 and 60 minutes after reperfusion. The tissue GSH content, in contrast, was decreased 60 minutes after reperfusion. Administration of fullerenol diminished these changes both preventively and therapeutically. Liver and renal functions were within normal limits in all groups. Moreover no obvious histopathologic additional damage could be found in either the P or the T group. It is suggested that fullerenol can be considered a powerful scavenger for the free radicals induced by UR injury of the small intestine.