Bioinformatic Analysis of Plasma Apolipoproteins A-I and A-II Revealed Unique Features of A-I/A-II HDL Particles in Human Plasma.
Bioinformatic Analysis of Plasma Apolipoproteins A-I and A-II Revealed Unique Features of A-I/A-II HDL Particles in Human Plasma.
复制标题
DOI：
10.1038/srep31532
复制
发表时间：
2016-08-16
影响因子：
4.6
通讯作者：
Yokoyama S
中科院分区：
文献类型：
--
作者：
Kido T;Kurata H;Kondo K;Itakura H;Okazaki M;Urata T;Yokoyama S
对314例日本受试者(男性177例，女性137例)的血浆apoA-I、apoA-II和apoA-II非相关apoA-I浓度进行了分析，其中包括1例(男性)纯合子和37例(20例男性，17例女性)CETP缺乏症杂合子。在野生型和杂合型CETP缺乏症患者中，不与apoA-II相关的apoA-I与apoA-II显著线性增加，而apoA-II仅轻微增加，且apoA-II相关的apoA-I与apoA-II的摩尔比保持在2不变。因此，总的高密度脂蛋白浓度几乎完全取决于含有apoA-I而不含apoA-II的高密度脂蛋白(LPAI)，而含有apoA-I和apoA-II的高密度脂蛋白(LPAI：AII)的浓度是恒定的，具有固定的摩尔比2：1，与总的高密度脂蛋白和载脂蛋白A-I的浓度无关。载脂蛋白A-I在LPAI和LPAI之间的分布：AII符合统计分配模型，与性别和CETP基因无关。分析还表明，LPA-I平均容纳4个apoA-I分子，清除率与LPAI：AII几乎没有区别。独立证据表明，LPAI：A-II的直径比LPAI小20%，与具有两个apoA-I和一个apoA-II的模型一致。这些粒子的功能贡献有待研究。
Plasma concentration of apoA-I, apoA-II and apoA-II-unassociated apoA-I was analyzed in 314 Japanese subjects (177 males and 137 females), including one (male) homozygote and 37 (20 males and 17 females) heterozygotes of genetic CETP deficiency. ApoA-I unassociated with apoA-II markedly and linearly increased with HDL-cholesterol, while apoA-II increased only very slightly and the ratio of apoA-II-associated apoA-I to apoA-II stayed constant at 2 in molar ratio throughout the increase of HDL-cholesterol, among the wild type and heterozygous CETP deficiency. Thus, overall HDL concentration almost exclusively depends on HDL with apoA-I without apoA-II (LpAI) while concentration of HDL containing apoA-I and apoA-II (LpAI:AII) is constant having a fixed molar ratio of 2 : 1 regardless of total HDL and apoA-I concentration. Distribution of apoA-I between LpAI and LpAI:AII is consistent with a model of statistical partitioning regardless of sex and CETP genotype. The analysis also indicated that LpA-I accommodates on average 4 apoA-I molecules and has a clearance rate indistinguishable from LpAI:AII. Independent evidence indicated LpAI:A-II has a diameter 20% smaller than LpAI, consistent with a model having two apoA-I and one apoA-II. The functional contribution of these particles is to be investigated.