Correlation of fetal DNA and human chorionic gonadotropin concentrations in second-trimester maternal serum.
Correlation of fetal DNA and human chorionic gonadotropin concentrations in second-trimester maternal serum.
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妊娠中期母体血清中胎儿 DNA 与人绒毛膜促性腺激素浓度的相关性。
作者：
Y. Ohashi;N. Miharu;H. Honda;O. Samura;K. Ohama
孕妇血清和血浆中游离胎儿DNA的发现为无创性产前诊断开辟了新途径，并为复杂妊娠提供了有用的标志物（1）（2）（3）（4）（5）（6）（7）。近年来，对母体血清或血浆中胎儿DNA的分析已经成功地提供了胎儿恒河猴D状态（2）和单基因疾病（3）以及胎儿性别（4）的产前诊断。此外，母体血清或血浆中胎儿DNA浓度增加已被证明是先兆子痫（5）（6）和早产（7）的有用标志物。然而，胎儿DNA的起源及其在复杂妊娠中增加的生物学意义仍不清楚。关于胎儿DNA的可能来源的推测包括有核胎儿细胞通过胎盘泄漏到母体循环中或滋养层DNA直接释放到母体循环中。人绒毛膜促性腺激素（hCG），这是由滋养细胞产生并直接分泌到母体循环，也据报道增加先兆子痫和早产（8）（9）（10）。本研究通过同时测量健康孕妇的胎儿DNA和hCG浓度来研究孕妇血清中胎儿DNA和hCG浓度之间的关系。
招募了63名健康孕妇（胎龄，15-17周），这些孕妇在广岛大学医院妇产科接受子宫穿刺术。在取得样本时，没有一名妇女表现出与怀孕有关的并发症，如产妇高血压或先兆流产。根据广岛大学研究伦理委员会批准的方案获得知情同意后，在穿刺前抽取12 mL肘前静脉血进行血清分离。立即将每份血样的6 mL等分试样以800 g离心...
The discovery of cell-free fetal DNA in maternal serum and plasma has opened a new avenue for noninvasive prenatal diagnosis and provided a useful marker of complicated pregnancy (1)(2)(3)(4)(5)(6)(7). In recent years, the analysis of fetal DNA in maternal serum or plasma has afforded successful prenatal diagnosis of fetal rhesus D status (2) and single-gene disorders (3), as well as fetal gender (4). In addition, an increased fetal DNA concentration in maternal serum or plasma has been demonstrated as a useful marker in preeclampsia (5)(6) and preterm labor (7). However, the origin of the fetal DNA and the biologic significance of its increase in complicated pregnancies remain unclear. Speculation as to the possible origin of fetal DNA has included the leakage of nucleated fetal cells into maternal circulation across the placenta or the direct release of trophoblast DNA into maternal circulation. Human chorionic gonadotropin (hCG), which is produced by trophoblasts and excreted directly into maternal circulation, is also reportedly increased in preeclampsia and preterm labor (8)(9)(10). The present study examined the association between fetal DNA and hCG concentrations in maternal serum by simultaneously measuring fetal DNA and hCG concentrations in healthy pregnant women.
Sixty-three healthy pregnant women (gestational age, 15–17 weeks) attending the Department of Obstetrics and Gynecology at Hiroshima University Hospital for amniocentesis were recruited. By the time samples were obtained, none of the women had manifested such pregnancy-related complications as maternal hypertension or threatened abortion. After informed consent was obtained under a protocol approved by the Research Ethics Committee of Hiroshima University, 12 mL of antecubital venous blood was drawn for serum separation just before amniocentesis. A 6-mL aliquot of each blood sample was immediately centrifuged at 800 g for …