Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways
Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways
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DOI：
10.1038/ncomms6305
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发表时间：
2014-10-01
影响因子：
16.6
通讯作者：
Ces, Oscar
中科院分区：
文献类型：
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作者：
Elani, Yuval;Law, Robert V.;Ces, Oscar
在自下而上合成生物学的学科中，囊泡定义了人工细胞的边界，并且越来越多地被用作在生理环境中操作的生化微反应器。随着该领域的成熟，有必要在囊泡内的不同空间位置划分过程，并使这些过程相互作用。在这里，我们通过设计和构建多隔室囊泡来解决这个问题，其中进行了工程化的多步酶促途径。各个步骤被隔离在不同的隔室中，并且它们的产物借助于跨膜蛋白孔穿过相邻的隔室，启动后续步骤。因此，在人工细胞系统中重建工程化的信号级联。重要的是，通过允许化学途径的不同步骤在空间上分离，该平台弥合了桌面化学和在囊泡内进行的化学之间的差距。
In the discipline of bottom-up synthetic biology, vesicles define the boundaries of artificial cells and are increasingly being used as biochemical microreactors operating in physiological environments. As the field matures, there is a need to compartmentalize processes in different spatial localities within vesicles, and for these processes to interact with one another. Here we address this by designing and constructing multi-compartment vesicles within which an engineered multi-step enzymatic pathway is carried out. The individual steps are isolated in distinct compartments, and their products traverse into adjacent compartments with the aid of transmembrane protein pores, initiating subsequent steps. Thus, an engineered signalling cascade is recreated in an artificial cellular system. Importantly, by allowing different steps of a chemical pathway to be separated in space, this platform bridges the gap between table-top chemistry and chemistry that is performed within vesicles.