A comparative evaluation of layer‐by‐layer assembly techniques for surface modification of microcarriers used in human mesenchymal stromal cell manufacturing
A comparative evaluation of layer‐by‐layer assembly techniques for surface modification of microcarriers used in human mesenchymal stromal cell manufacturing
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DOI：
10.1002/biot.202100605
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发表时间：
2022-04
影响因子：
4.7
通讯作者：
Hemanta Timsina;J. McTyer;R. Rao;Jorge Almodóvar
中科院分区：
文献类型：
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作者：
Hemanta Timsina;J. McTyer;R. Rao;Jorge Almodóvar
鉴于其治疗潜力，对大量高效人间充质基质细胞（hMSCs）的需求正在增长。为了满足高产量需求，通常使用使用微载体的悬浮细胞培养。微载体通常由细胞外基质蛋白或带电化合物制成或涂覆有细胞外基质蛋白或带电化合物以促进细胞粘附和增殖。在这项工作中，一个简单的方法（排水过滤器）进行逐层（LbL）组装微载体上创建肝素和胶原蛋白的多层，并进一步证明，这些多层培养48小时后的hMSC的活力有积极的影响被证明。与文献中发现的另外两种方法-离心法和流化床法相比，排水过滤法可以更有效地进行表面改性，并且在涂覆过程中所需的材料和步骤更少。
The demand for large quantities of highly potent human mesenchymal stromal cells (hMSCs) is growing given their therapeutic potential. To meet high production needs, suspension‐based cell cultures using microcarriers are commonly used. Microcarriers are commonly made of or coated with extracellular matrix proteins or charged compounds to promote cell adhesion and proliferation. In this work, a simple method (draining filter) to perform layer by layer (LbL) assembly on microcarriers to create multilayers of heparin and collagen and further demonstrate that these multilayers have a positive effect on hMSC viability after 48 h of culture was demonstrated. The draining filter method is evaluated against two other methods found in literature—centrifugation and fluidized bed, showing that the draining filter method can perform the surface modification with greater efficiency and with less materials and steps needed in the coating process.