A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay.
A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay.
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DOI：
10.1128/jcm.02166-16
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发表时间：
2017-03
影响因子：
9.4
通讯作者：
Langford PR
中科院分区：
文献类型：
--
作者：
Bossé JT;Li Y;Sárközi R;Gottschalk M;Angen Ø;Nedbalcova K;Rycroft AN;Fodor L;Langford PR
胸膜肺炎放线杆菌可引发胸膜肺炎，这是一种对养猪业具有重大经济影响的肺部疾病。近期，有研究描述了一些胸膜肺炎放线杆菌的分离株，它们在血清学上不同于先前已确定特征的15种血清型，并且有人提出这些分离株构成了一个新的血清型，即血清型16。在此，我们对所提出的血清型16参考菌株A - 85/14进行了全基因组测序，以确认存在一个独特的荚膜多糖生物合成位点。为了进行分子诊断，我们根据菌株A - 85/14的荚膜位点设计了引物，并制定了一种聚合酶链式反应（PCR）方法，该方法能够将血清型16的分离株与所有15种已知血清型以及猪的其他常见呼吸道致病/共生细菌区分开来。对菌株A - 85/14的荚膜位点分析结合先前的血清学数据表明，胸膜肺炎放线杆菌存在第16种血清型，即血清型16。
Actinobacillus pleuropneumoniae causes pleuropneumonia, an economically significant lung disease of pigs. Recently, isolates of A. pleuropneumoniae that were serologically distinct from the previously characterized 15 serovars were described, and a proposal was put forward that they comprised a new serovar, serovar 16. Here we used whole-genome sequencing of the proposed serovar 16 reference strain A-85/14 to confirm the presence of a unique capsular polysaccharide biosynthetic locus. For molecular diagnostics, primers were designed from the capsule locus of strain A-85/14, and a PCR was formulated that differentiated serovar 16 isolates from all 15 known serovars and other common respiratory pathogenic/commensal bacteria of pigs. Analysis of the capsule locus of strain A-85/14 combined with the previous serological data show the existence of a sixteenth serovar—designated serovar 16—of A. pleuropneumoniae.