AtBI-1, a plant homologue of Bax inhibitor-1, suppresses Bax-induced cell death in yeast and is rapidly upregulated during wounding and pathogen challenge.
AtBI-1, a plant homologue of Bax inhibitor-1, suppresses Bax-induced cell death in yeast and is rapidly upregulated during wounding and pathogen challenge.
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DOI：
10.1046/j.1365-313x.2000.00690.x
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发表时间：
2000-02
期刊：
影响因子：
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通讯作者：
Pedro Sanchez;Marta de Torres Zabala;Murray R Grant
中科院分区：
文献类型：
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作者：
Pedro Sanchez;Marta de Torres Zabala;Murray R Grant
广泛的搜索迄今未能确定哺乳动物细胞凋亡机制的功能植物同源物。在这里，我们报告的分离和表征的拟南芥同源物的人类Bax抑制剂-1，AtBI-1，分离过程中的差异筛选植物病原体假单胞菌的挑战。AtBI是拟南芥中的一个小基因家族的成员，其成员与人BI-1具有广泛的氨基酸同一性。AtBI-1在抑制酵母中由哺乳动物促凋亡蛋白Bax表达所赋予的致死表型的能力方面也与BI-1功能相似。AtBI-1的表达在植物受伤或病原体的挑战过程中迅速上调，这表明在生物和非生物胁迫的反应中的作用。AtBI-1的上调表现为不依赖于R基因，并且不受特定类型的R基因所需突变的显著影响。然而，AtBI-1信息的积累在coi 1中显著减少，其中对昆虫、病原体和创伤的防御反应受到损害。
Extensive searches have so far failed to identify functional plant homologues of the mammalian apoptotic machinery. Here we report the isolation and characterisation of an Arabidopsis thaliana homologue of human Bax Inhibitor-1, AtBI-1, isolated during a differential screen of plants challenged with the phytopathogen Pseudomonas syringae. AtBI is a member of a small gene family in Arabidopsis, members of which display extensive amino acid identity to human BI-1. AtBI-1 is also functionally similar to BI-1 in its ability to suppress the lethal phenotype in yeast conferred by expression of the mammalian proapoptotic protein, Bax. Expression of AtBI-1 is rapidly upregulated in plants during wounding or pathogen challenge, suggesting a role in responses to biotic and abiotic stress. AtBI-1 upregulation appears R gene independent and is not markedly affected by mutations required for specific classes of R genes. However, the accumulation of AtBI-1 message is significantly reduced in coi1, in which defence responses to insects, pathogens and wounding are compromised.