Expression of int-2 mRNA in human tumors amplified at the int-2 locus.
Expression of int-2 mRNA in human tumors amplified at the int-2 locus.
复制标题
人类肿瘤中 int-2 mRNA 的表达在 int-2 基因座处扩增。
作者：
D. Liscia;G. Merlo;C. Garrett;D. French;R. Mariani;R. Callahan
基因扩增在人类恶性肿瘤中是一种相对频繁的事件，被认为在肿瘤转化和肿瘤进展中具有重要作用。我们的注意力集中在INT-2基因的扩增和表达上，原因有几个：(1)在小鼠乳腺肿瘤发生过程中，INT-2经常被MMTV前病毒整合激活。(2)人类INT-2同源基因位于染色体11q13上，在人类原发肿瘤中经常被扩增，并且包含hst基因的扩增单位，hst基因通常是共扩增的；在乳腺癌中，11q13基因的扩增与疾病的不良预后有关。(3)int-2和hst属于基本的成纤维细胞生长因子基因家族。所有这些观察都提出了人类INT-2基因在肿瘤过程中发挥积极作用的可能性，但只有在INT-2在人类肿瘤中表达的情况下，才能证明这一点。在本研究中，我们利用RNA：RNA原位杂交和Northern印迹分析表明，在同一基因座扩增的多个人类肿瘤中都有INT-2基因的表达。
Gene amplification is a relatively frequent event in human malignant tumors and is believed to have an important function in neoplastic transformation and tumor progression. Our attention has been focused on the amplification and the expression of the int-2 gene for several reasons: (1) In the mouse mammary tumorigenesis int-2 is frequently activated by MMTV proviral integration. (2) The human homolog of int-2, located on chromosome 11q13, is frequently amplified in human primary tumors and is comprised in an amplification unit encompassing the hst gene, which is often coamplified; the amplification at the 11q13 locus in breast carcinomas correlates with a poor outcome of the disease. (3) int-2 and hst belong to the basic FGF gene family. All these observations raise the possibility that the human int-2 gene plays an active role in the neoplastic process, but this will prove to be true only if int-2 is expressed in human tumors. In the present study we used RNA:RNA in situ hybridization and Northern blot analysis to show that int-2 gene is expressed in a number of human carcinomas amplified at the same locus.