Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium
Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium
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Ezh2在发育中的小鼠耳蜗感觉上皮中的时空表达
DOI：
10.1007/s11684-016-0459-6
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发表时间：
2016-09-01
影响因子：
8.1
通讯作者：
Li, Huawei
中科院分区：
文献类型：
--
作者：
Chen, Yan;Li, Wenyan;Li, Huawei
zeste 2多梳抑制复合物2亚基（Ezh 2）的增强子是一种参与DNA甲基化的组蛋白-赖氨酸N甲基转移酶。Ezh 2也被报道在干细胞增殖和分化中起关键作用。然而，Ezh 2在小鼠耳蜗发育过程中的详细表达谱尚未研究。在这里，我们研究了Ezh 2在胚胎和出生后发育过程中的耳蜗时空表达。Ezh 2在胚胎9.5天（E9.5）开始在整个耳囊核中表达。在E12.5，Ezh 2在耳蜗前感觉上皮细胞核中表达。在E13.5和E15.5，Ezh 2在分化中的耳蜗上皮细胞核中从顶向底转表达。在出生后0天和7天，Ezh 2的表达位于耳蜗上皮细胞的核中，在所有三个转弯，可以清楚地看到外毛细胞和内毛细胞，支持细胞，血管纹和螺旋神经节细胞。Ezh 2在成年小鼠耳蜗上皮中持续表达。我们的研究结果提供了基本的Ezh 2表达模式，并可能有助于进一步研究Ezh 2在耳蜗发育过程中的详细作用。
The enhancer of zeste 2 polycomb repressive complex 2 subunit (Ezh2) is a histone-lysine Nmethyltransferase enzyme that participates in DNA methylation. Ezh2 has also been reported to play crucial roles in stem cell proliferation and differentiation. However, the detailed expression profile of Ezh2 during mouse cochlear development has not been investigated. Here, we examined the spatiotemporal expression of Ezh2 in the cochlea during embryonic and postnatal development. Ezh2 expression began to be observed in the whole otocyst nuclei at embryonic day 9.5 (E9.5). At E12.5, Ezh2 was expressed in the nuclei of the cochlear prosensory epithelium. At E13.5 and E15.5, Ezh2 was expressed from the apical to the basal turns in the nuclei of the differentiating cochlear epithelium. At postnatal day (P) 0 and 7, the Ezh2 expression was located in the nuclei of the cochlear epithelium in all three turns and could be clearly seen in outer and inner hair cells, supporting cells, the stria vascularis, and spiral ganglion cells. Ezh2 continued to be expressed in the cochlear epithelium of adult mice. Our results provide the basic Ezh2 expression pattern and might be useful for further investigating the detailed role of Ezh2 during cochlear development.