RAT CILIARY NEUROTROPHIC FACTOR (CNTF) - GENE STRUCTURE AND REGULATION OF MESSENGER-RNA LEVELS IN GLIAL-CELL CULTURES
RAT CILIARY NEUROTROPHIC FACTOR (CNTF) - GENE STRUCTURE AND REGULATION OF MESSENGER-RNA LEVELS IN GLIAL-CELL CULTURES
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DOI：
10.1002/glia.440090303
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发表时间：
1993-11-01
期刊：
影响因子：
6.2
通讯作者：
THOENEN, H
中科院分区：
文献类型：
--
作者：
CARROLL, P;SENDTNER, M;THOENEN, H
研究了大鼠睫状神经营养因子（CNTF）基因的结构以及培养的神经胶质细胞中CNTF mRNA水平的调节。大鼠 mRNA 由简单的双外显子转录单元编码。对转录起始位点上游区域的序列分析没有揭示典型的 TATA-box 共有序列。在培养的施万细胞中检测到低水平的CNTF mRNA，并且多种处理均未增加CNTF mRNA。来自新生大鼠大脑的三周龄富含星形胶质细胞的细胞培养物含有易于检测到的 CNTF mRNA。在富含星形胶质细胞的培养物中，用 IFN-γ 处理后观察到 CNTF mRNA 水平上调。通过提高细胞内环磷酸腺苷的治疗和成纤维细胞生长因子 (FC;F) 家族成员的治疗，这些细胞中 CNTF mRNA 水平下调。讨论了这些结果对 CNTF 潜在体内功能的影响。 (C) 1993 Wiley-Liss, Inc.
The structure of the rat ciliary neurotrophic factor (CNTF) gene and the regulation of CNTF mRNA levels in cultured glial cells were investigated. The rat mRNA is encoded by a simple two-exon transcription unit. Sequence analysis of the region upstream of the transcription start-site did not reveal a typical TATA-box consensus sequence. Low levels of CNTF mRNA were detected in cultured Schwann cells, and CNTF mRNA was not increased by a variety of treatments. Three-week-old astrocyte-enriched cell cultures from new-born rat brain contained easily detectable CNTF mRNA. In astrocyte-enriched cultures, upregulation of CNTF mRNA levels was observed after treatment with IFN-gamma. CNTF mRNA levels were down-regulated in these cells by treatments that elevate intracellular cyclic AMP and by members of the fibroblast growth factor (FC;F) family. The implications of these results for potential in vivo functions of CNTF are discussed. (C) 1993 Wiley-Liss, Inc.