T cells within germinal centers are specific for the immunizing antigen.
T cells within germinal centers are specific for the immunizing antigen.
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DOI：
10.4049/jimmunol.151.9.4505
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发表时间：
1993-11
影响因子：
4.4
通讯作者：
K. Fuller;O. Kanagawa;M. Nahm
中科院分区：
文献类型：
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作者：
K. Fuller;O. Kanagawa;M. Nahm
对T细胞依赖性Ag的正常抗体应答需要Ag特异性B和T细胞之间的物理接触。由于这种Ag特异性细胞在体内是罕见的，我们试图确定它们彼此物理接触的体内部位。我们检测了淋巴结中生发中心（GC）中T细胞的Ag特异性，已知Ag特异性B细胞在GC中增殖和成熟。我们通过检测两个特征原位研究了GC T细胞的Ag特异性：1）某些V α和V β TCR家族的表达; 2）暴露于Ag后，溴脱氧尿苷掺入T细胞DNA作为Ag诱导增殖的指标。当用细胞色素c和髓鞘碱性蛋白在小鼠中诱导GC时，发现GC T细胞优先表达V α 11和V β 8 TCR家族，它们分别是这两种应答中的优势TCR家族。此外，GC T细胞在暴露于诱导GC形成的Ag后增殖。总而言之，这些结果表明GC必须招募和保留Ag特异性T细胞，从而暗示GC是Ag特异性T细胞和B细胞相互作用的体内位点。
A normal antibody response to T cell-dependent Ag requires physical contact between Ag-specific B and T cells. Because such Ag-specific cells are rare in vivo, we sought to identify an in vivo site where they physically contact each other. We examined the Ag specificity of T cells in germinal centers (GC) in lymph nodes, where it is known that Ag-specific B cells proliferate and mature. We investigated the Ag specificity of GC T cells in situ by examining two characteristics: 1) expression of certain V alpha and V beta TCR families; and 2) incorporation of bromodeoxyuridine into T cell DNA after exposure to Ag as an index of Ag-induced proliferation. When GC were induced in mice with cytochrome c and myelin basic protein, the GC T cells were found to preferentially express V alpha 11 and V beta 8 TCR families, which are, respectively, the dominant TCR families in these two responses. Furthermore, GC T cells have proliferated upon exposure to the Ag that induced GC formation. Taken together, these results demonstrate that GC must recruit and retain Ag-specific T cells, thus implicating the GC as an in vivo site where Ag-specific T and B cells interact.