Induction of avian serum apolipoprotein II and vitellogenin by tamoxifen.
Induction of avian serum apolipoprotein II and vitellogenin by tamoxifen.
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他莫昔芬诱导禽血清载脂蛋白 II 和卵黄蛋白原。
DOI：
10.1016/0006-291x(81)91180-3
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发表时间：
1981
影响因子：
3.1
通讯作者：
Williams,DL
中科院分区：
文献类型：
--
作者：
Blue,ML;Williams,DL
用放射免疫分析法检测了未雌激素处理公鸡血清中的载脂蛋白Ⅱ和卵黄蛋白原。三苯氧胺（30 mg/kg）可使基础血清载脂蛋白Ⅱ（apo Ⅱ）升高50倍以上，卵黄蛋白原（vitellogenin）升高15倍以上。血清中载脂蛋白II的积累是双相的增加剂量的他莫昔芬。这种双相反应反映在肝脏载脂蛋白II合成的相对速率中。他莫昔芬代谢产物羟基他莫昔芬和去甲基他莫昔芬以及三苯乙烯类抗雌激素CI 628也可增加血清载脂蛋白II。这些研究表明，卵黄蛋白原和载脂蛋白II基因的表达发生在基础率外源性雌激素治疗前。这些实验还表明，当使用非常敏感的技术来测量雌激素调节蛋白时，三苯乙烯类抗雌激素在鸡中具有激动剂活性。
Apo II and vitellogenin were detected in sera of nonestrogenized roosters by radioimmunoassay. Tamoxifen (30mg/kg) raised basal serum apo II more than 50 fold and vitellogenin more than 15 fold. Serum accumulation of apo II was biphasic in response to increasing doses of tamoxifen. This biphasic response was reflected in the relative rates of hepatic apo II synthesis. The tamoxifen metabolites, hydroxytamoxifen and desmethyltamoxifen, and the triphenylethylene antiestrogen, CI 628, also increased serum apo II. These studies show that the expression of vitellogenin and apo II genes occurs at a basal rate prior to exogenous estrogen treatment. These experiments also demonstrate that the triphenylethylene antiestrogens have agonist activity in the chicken when very sensitive techniques are used to measure estrogen-regulated proteins.