High-Temperature-Responsive Poplar lncRNAs Modulate Target Gene Expression via RNA Interference and Act as RNA Scaffolds to Enhance Heat Tolerance.
High-Temperature-Responsive Poplar lncRNAs Modulate Target Gene Expression via RNA Interference and Act as RNA Scaffolds to Enhance Heat Tolerance.
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高温响应性杨树 lncRNA 通过 RNA 干扰调节靶基因表达并作为 RNA 支架增强耐热性
DOI：
10.3390/ijms21186808
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发表时间：
2020-09-16
影响因子：
5.6
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Song Y;Chen P;Liu P;Bu C;Zhang D
高温胁迫对植物的生长发育和生存是一种威胁。长非编码RNA(LncRNAs)参与植物的逆境反应，但它们在复杂的逆境反应网络中的功能尚不清楚。杨树对陆地生态稳定有贡献。在本研究中，我们利用链特异RNA测序技术(ssRNA-seq)，在一个非生物耐受胁迫的杨树物种中鉴定了204个高温响应的lncRNAs。通过模拟杨树中过表达和抑制的候选LncRNAs，利用纳米薄膜介导法阐明其在靶标上的调控模式。这些LncRNAs被预测为针对185个基因，其中100个是顺式基因，119个是反式基因。基因本体富集化分析表明，该基因的解剖结构、形态发生以及对胁迫和信号的响应都得到了显着的丰富。在热敏感的lncRNA中，Tons_00202587通过其二级结构与上游序列结合，干扰靶基因的转录。TCONS_00260893通过干扰靶基因的特定变异体/异构体来促进高温处理下的钙内流。这两个lncRNA靶标的异源表达促进了热胁迫下拟南芥的光合作用保护和恢复，抑制了膜过氧化，抑制了DNA损伤。这些结果表明，lncRNAs可以作为潜在的RNA支架或通过RNA干扰途径来调节其靶基因。
High-temperature stress is a threat to plant development and survival. Long noncoding RNAs (lncRNAs) participate in plant stress responses, but their functions in the complex stress response network remain unknown. Poplar contributes to terrestrial ecological stability. In this study, we identified 204 high-temperature-responsive lncRNAs in an abiotic stress-tolerant poplar (Populus simonii) species using strand-specific RNA sequencing (ssRNA-seq). Mimicking overexpressed and repressed candidate lncRNAs in poplar was used to illuminate their regulation pattern on targets using nano sheet mediation. These lncRNAs were predicted to target 185 genes, of which 100 were cis genes and 119 were trans genes. Gene Ontology enrichment analysis showed that anatomical structure morphogenesis and response to stress and signaling were significantly enriched. Among heat-responsive LncRNAs, TCONS_00202587 binds to upstream sequences via its secondary structure and interferes with target gene transcription. TCONS_00260893 enhances calcium influx in response to high-temperature treatment by interfering with a specific variant/isoform of the target gene. Heterogeneous expression of these two lncRNA targets promoted photosynthetic protection and recovery, inhibited membrane peroxidation, and suppressed DNA damage in Arabidopsis under heat stress. These results showed that lncRNAs can regulate their target genes by acting as potential RNA scaffolds or through the RNA interference pathway.
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影响因子：
7.4
作者：
He, Jiali;Li, Hong;Luo, Zhi-Bin
通讯作者：
Luo, Zhi-Bin
影响因子：
10.5
作者：
Cabili, Moran N.;Trapnell, Cole;Rinn, John L.
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模因套件：用于发现和搜索的工具。
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DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
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通讯作者：
HOCHBERG, Y
影响因子：
14.9
作者：
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