Ultrasound contrast-enhanced imaging and in vitro antitumor effect of paclitaxel-poly(lactic-co-glycolic acid)-monomethoxypoly (ethylene glycol) nanocapsules with ultrasound-targeted microbubble destruction.
Ultrasound contrast-enhanced imaging and in vitro antitumor effect of paclitaxel-poly(lactic-co-glycolic acid)-monomethoxypoly (ethylene glycol) nanocapsules with ultrasound-targeted microbubble destruction.
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紫杉醇-聚(乳酸-乙醇酸)-单甲氧基聚(乙二醇)纳米胶囊的超声对比增强成像和体外抗肿瘤作用与超声靶向微泡破坏。
DOI：
10.3892/mmr.2014.3072
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发表时间：
2015-04
影响因子：
3.4
通讯作者：
Du LF
中科院分区：
文献类型：
--
作者：
Ma J;Xing LX;Shen M;Li F;Zhu MJ;Jin LF;Li Z;Gao F;Su Y;Duan YR;Du LF
A combination of diagnostic and therapeutic ultrasound (US) techniques may be able to provide the basis of specific therapeutic protocols, particularly for the treatment of tumors. Nanotechnology may aid the progression towards the use of US for tumor diagnosis and targeted therapy. The current study investigated in vivo and in vitro US contrast imaging using nanocapsules (NCs), and also US and US-targeted microbubble destruction (UTMD) therapy using drug-loaded NCs for pancreatic cancer in vitro. In the current study, the NCs were made from the polymer nanomaterial poly(lactic-co-glycolic acid)-monomethoxypoly(ethylene glycol) (PLGA-mPEG), encapsulated with paclitaxel (PTX), to create PTX-PLGA-mPEG NCs. The PTX-PLGA-mPEG NCs were used as a US contrast agent (UCA), which produced satisfactory US contrast-enhanced images in vitro and in vivo of the rabbit kidneys, with good contrast compared with lesions in the peripheral regions. However, clear contrast-enhanced images were not obtained using PTX-PLGA-mPEG NCs as a UCA, when imaging the superficial pancreatic tumors of nude mice in vivo. Subsequently, fluorescence and flow cytometry were used to measure the NC uptake rate of pancreatic tumor cells under various US or UTMD conditions. An MTT assay was used to evaluate the efficiency of PTX and PTX-PLGA-mPEG NCs in killing tumor cells following 24 or 48 h of US or UTMD therapy, compared with controls. The specific US or UTMD conditions had been previously demonstrated to be optimal through repeated testing, to determine the conditions by which cells were not impaired and the efficiency of uptake of nanoparticles was highest. The current study demonstrated high cellular uptake rates of PLGA-mPEG NCs and high tumor cell mortality with PTX-PLGA-mPEG NCs under US or UTMD optimal conditions. It was concluded that the use of NCs in US-mediated imaging and antitumor therapy may provide a novel application for US.
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影响因子：
2.9
作者：
Miller, DL;Gies, RA
通讯作者：
Gies, RA
基于由 Span 60 和聚氧乙烯 40 硬脂酸酯的表面活性剂混合物产生的微泡的新型超声造影剂。
影响因子：
9.7
作者：
Xing, Zhanwen;Ke, Hengte;Liu, Jibin
通讯作者：
Liu, Jibin
影响因子：
9.9
作者：
Chen, Min;Cooper, Helen M.;Xu, Zhi Ping
通讯作者：
Xu, Zhi Ping
影响因子：
39.3
作者：
Vancraeynest, D;Havaux, X;Vanoverschelde, JLJ
通讯作者：
Vanoverschelde, JLJ
影响因子：
3.5
作者：
Du, Jing;Shi, Qiu Sheng;Duan, You Rong
通讯作者：
Duan, You Rong