Alpha 1-proteinase inhibitor is more sensitive to inactivation by cigarette smoke than is leukocyte elastase.
Alpha 1-proteinase inhibitor is more sensitive to inactivation by cigarette smoke than is leukocyte elastase.
复制标题
与白细胞弹性蛋白酶相比，α1-蛋白酶抑制剂对香烟烟雾的失活更为敏感。
DOI：
10.1164/arrd.1982.126.4.691
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Dearing,R
中科院分区：
文献类型：
--
作者：
Janoff,A;Dearing,R
将气相香烟烟雾的水溶液与纯人类白细胞弹性蛋白酶或与粗人类白细胞颗粒提取物一起孵育，并使用合成酰胺底物测定对酶活性的影响。同时，在相同的条件下，将相同的烟雾溶液与10%人血清一起孵育，并类似地测量对纯化的或粗制的白细胞弹性蛋白酶的血清抑制的影响。此外，将未分级香烟烟雾的水溶液与白细胞弹性蛋白酶或血清一起孵育，并测定烟雾处理的酶消化弹性蛋白和烟雾处理的血清抑制弹性蛋白消化的能力。两个实验方案表明，血清弹性蛋白酶抑制能力（主要由α 1-蛋白酶抑制剂引起）比白细胞弹性蛋白酶更容易被香烟烟雾的水溶液灭活，这表明弹性蛋白酶抑制（而不是弹性蛋白酶活性）可能主要被香烟烟雾吸入体内抑制。
Aqueous solutions of gas phase cigarette smoke were incubated with pure human leukocyte elastase or with crude human leukocyte granule extract, and the effects on enzyme activity were determined using a synthetic amide substrate. Simultaneously, the same smoke solutions were incubated with 10% human serum under identical conditions, and the effects on serum inhibition of purified or crude leukocyte elastase were similarly measured. In addition, aqueous solutions of unfractionated cigarette smoke were incubated with leukocyte elastase or serum, and the abilities of the smoke-treated enzyme to digest elastin and of the smoke-treated serum to inhibit elastin digestion were determined. Both experimental protocols showed that serum elastase-inhibiting capacity (primarily caused by alpha1-proteinase inhibitor) is more susceptible to inactivation by aqueous solutions of cigarette smoke than is leukocyte elastase, suggesting that elastase inhibition (rather than elastase activity) may be predominantly suppressed by cigarette smoke inhalationin vivo.