Mouse calbindin-D9k gene expression in the uterus during late pregnancy and lactation
Mouse calbindin-D9k gene expression in the uterus during late pregnancy and lactation
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DOI：
10.1016/s0303-7207(03)00203-x
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发表时间：
2003-07-31
影响因子：
4.1
通讯作者：
Jeung, EB
中科院分区：
文献类型：
--
作者：
An, BS;Choi, KC;Jeung, EB
钙结合蛋白-D-9k (CaBP-9k) 是一种胞质钙结合蛋白，主要在十二指肠、胎盘和子宫中表达。为了了解子宫CaBP-9k基因的表达模式和调控，我们研究了妊娠后期（第12天至18天）和哺乳期小鼠子宫中CaBP-9k mRNA的表达及其对雌激素（E2）和孕激素（P4）的调节。通过 Northern blot 分析测量子宫 CaBP-9k、雌激素受体 α (ERα) 和孕激素受体 (PR) mRNA 的表达水平。小鼠子宫CaBP-9k mRNA的表达水平从妊娠第16天（P16）开始逐渐增加，在P18时达到峰值（是P12的6.0倍），并在出生时和哺乳期间下降。 ERα和PR mRNA的表达水平显示与CaBP-9k mRNA相似的波动，表明性类固醇/受体在CaBP-9k基因的调节中的作用。为了研究类固醇激素对 CaBP-9k mRNA 表达的影响，三组动物分别注射（皮下）类固醇激素拮抗剂（RU486、他莫昔芬和 IC1182780）。 RU486 是一种 P4 拮抗剂，在 48 小时（3.2 倍）和 72 小时（3.8 倍）时诱导 CaBP-9k mRNA 表达显着降低。然而，他莫昔芬和 IC1182780（E2 拮抗剂）对 CaBP-9k mRNA 表达没有影响。与RU486处理相比，48小时和72小时时，RU486和ICI182780联合处理并没有进一步降低CaBP-9k mRNA的表达水平。此外，使用糖皮质激素/黄体酮拮抗剂 RU40555 治疗后，It 值分别下降 48 和 72。这些结果表明E2不太可能参与妊娠后期和哺乳期小鼠子宫CaBP-9k基因的调节。总之，目前的结果表明 P4，而不是 E2，是妊娠晚期和哺乳期 CaBP-9k mRNA 表达的关键调节因子。 (C) 2003 Elsevier Ireland Ltd. 保留所有权利。
Calbindin-D-9k (CaBP-9k) is a cytosolic calcium binding protein mainly expressed in duodenum, placenta and uterus. In order to understand the expression pattern and regulation of uterine CaBP-9k gene, the expression of CaBP-9k mRNA and its regulation by estrogen (E2) and progesterone (P4) were investigated in the mouse uterus during late pregnancy (from day 12 to 18) and lactation. The expression levels of uterine CaBP-9k, estrogen receptor alpha (ERalpha) and progesterone receptor (PR) mRNAs were measured by Northern blot analysis. The expression levels of mouse uterine CaBP-9k mRNA gradually increased from pregnancy day 16 (P16), peaked at P18 (6.0-fold vs. P12) and declined at birth and during lactation. The expression levels of ERalpha and PR mRNAs indicated a similar fluctuation as CaBP-9k mRNA, suggesting the role of sex steroids/receptors in the regulation of CaBP-9k gene. To investigate effect of steroid hormone on CaBP-9k mRNA expression, three groups of animals were injected (s.c) with steroid hormone antagonists (RU486, tamoxifen, and IC1182780), respectively. RU486, a P4 antagonist, induced a significant decrease in CaBP-9k mRNA expression at 48 (3.2-fold) and 72 h (3.8-fold). However, tamoxifen and IC1182780, E2 antagonists, had no effect on CaBP-9k mRNA expression. Combined treatment with RU486 and ICI182780 did not further decrease the expression level of CaBP-9k mRNA when compared with RU486 treatment at 48 and 72 h. In addition, the treatment with RU40555, a glucocorticoid/progesterone antagonist, resulted in a decrease at 48 and 72 It following treatment. These results indicate that E2 is not likely involved in the regulation of CaBP-9k gene in the mouse uterus during late pregnancy and lactation. In conclusion, the present results suggest that P4, not E2 is a key regulator of CaBP-9k mRNA expression during late pregnancy and lactation. (C) 2003 Elsevier Ireland Ltd. All rights reserved.