Expression of a RING-HC protein from rice improves resistance to Pseudomonas syringae pv. tomato DC3000 in transgenic Arabidopsis thaliana
Expression of a RING-HC protein from rice improves resistance to Pseudomonas syringae pv. tomato DC3000 in transgenic Arabidopsis thaliana
复制标题
DOI：
10.1093/jxb/erm272
复制
发表时间：
2007-01-01
影响因子：
6.9
通讯作者：
Lam, Hon-Ming
中科院分区：
文献类型：
--
作者：
Cheung, Ming-Yan;Zeng, Nai-Yan;Lam, Hon-Ming
获得了一个cDNA克隆（OsRHC1），该克隆编码了一个新的RING锌指蛋白，其结构特征与一组来自单子叶和双子叶的密切相关的带注释蛋白相似（n -一半有多个跨膜结构域；在C端有一个独特的RING锌指共识CYS-X-2-CYS-X-11-Cys-X-HiS-X-3-CYS-X-2-CYS-X-6-CYS-X-2-CYS）。OsRHC1定位于水稻细胞质膜上，在含有Xa位点的水稻品系中通过损伤诱导。水稻OsRHC1基因在拟南芥（拟南芥）中的生态表达增强了对丁香假单胞菌的防御反应。表明OsRHC1可能具有广谱抗病能力。在MG132存在或NPR1 -3突变背景下，保护作用被中和，这表明OsRHC1的功能依赖于泛素介导的蛋白降解，通过26S蛋白酶体和关键防御反应调节因子NPR1的存在。
A cDNA clone (OsRHC1) was obtained, which encodes a novel RING zinc finger protein sharing similar structural features (multiple transmembrane domains at the N-half; a unique RING zinc finger consensus CYS-X-2-CYS-X-11-Cys-X-HiS-X-3-CYS-X-2-CYS-X-6-CYS-X-2-CYS at the C terminus) to a group of closely related annotated proteins from both monocots and dicots. OsRHC1 was found to be localized on plasma membrane of rice cells and induced by wounding in rice lines containing Xa loci. Ecotopic expression of the OsRHC1 cDNA from rice (a monocot) in transgenic Arabidopsis thaliana (a dicot) enhanced the defence response toward Pseudomonas syringae pv. tomato DC3000, suggesting that OsRHC1 may confer broad-spectrum disease resistance. The protective effects were neutralized in the presence of MG132 or in an npr1-3 mutation background, indicating that the function of OsRHC1 is dependent on the ubiquitin-mediated protein degradation via the 26S proteasome and the presence of the key defence response regulator NPR1.