In vitro cellular uptake and cytotoxic effect of functionalized nickel nanoparticles on leukemia cancer cells
In vitro cellular uptake and cytotoxic effect of functionalized nickel nanoparticles on leukemia cancer cells
复制标题
功能化镍纳米颗粒对白血病癌细胞的体外细胞摄取和细胞毒作用。
DOI：
10.1166/jnn.2008.311
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发表时间：
2008-05-01
影响因子：
--
通讯作者：
Chen, Baoan
中科院分区：
文献类型：
--
作者：
Guo, Dadong;Wu, Chunhui;Chen, Baoan
纳米镍以其独特的结构和性能，如催化剂、高密度磁记录介质等，得到了广泛的应用。然而，它们在生物学上的应用很少，Ni NPs对生物系统的具体影响仍然未知。本研究探讨了磁性Ni NPs在癌细胞研究中应用的可能性，并研究了带正电的四庚基铵包封的Ni NPs对体外白血病K562细胞的作用。通过光学显微镜、原子力显微镜（AFM）和扫描电镜（SEM）的观察，可以明显观察到Ni NPs诱导癌细胞的形态学变化。3-（4,5-二甲基噻唑-2-酰基）2,5-二苯基溴化四唑（MTT）实验、DNA片段化和流式细胞术研究结果表明，Ni NPs在高浓度时对白血病K562细胞具有细胞毒性，可诱导靶癌细胞凋亡和坏死，而在低浓度时对靶细胞影响不大。同时，带正电荷基团的功能化Ni NPs可以增强细胞膜的通透性，促进细胞将外部靶分子摄取到癌细胞中。这些发现揭示了Ni NPs靶向癌细胞并诱导白血病细胞毒性的潜在机制，为Ni NPs在相关临床和生物医学领域的应用提供了可能。
Nickel nanoparticles (Ni NPs) have been applied in a wide range of areas because of their unique structure and properties such as catalysts, high-density magnetic recording media and others. However, little effort has been paid to their biological application and the concrete effect of Ni NPs on biological systems is still unknown. In this study, the possibility of the utilization of the magnetic Ni NPs in cancer cell studies was explored and the effects of the Ni NPs capped with positively charged tetraheptylammonium on leukemia K562 cells in vitro were investigated. Our observations of optical microscopy, atomic force microscopy (AFM) and scanning electron microscopy (SEM) studies indicate that the morphological changes of cancer cells induced by Ni NPs could be apparently observed. The results of 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay, DNA fragmentation and flow cytometry studies demonstrate that the Ni NPs could exert cytotoxicity to leukemia K562 cells at high concentration, and subsequently induce both apoptosis and necrosis of target cancer cells, whilst it had little impact on target cells when at low concentration. Meanwhile, functionalized Ni NPs with positively charged groups could enhance the permeability of cell membrane and facilitate the cellular uptake of outer target molecules into cancer cells. These findings reveal the potential mechanism of Ni NPs to target cancer cells which could induce the cytotoxicity to leukemia cancer cells and suggest the possibility for applications of the Ni NPs in related clinical and biomedical areas.