An enzymatic tissue digestion method for fibre biopersistence studies
An enzymatic tissue digestion method for fibre biopersistence studies
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DOI：
10.1016/s0003-4878(97)00023-9
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发表时间：
1997-12-01
影响因子：
--
通讯作者：
Cullen, RT
中科院分区：
文献类型：
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作者：
Searl, A;Cullen, RT
INTRODUCTION Measurement of the biopersistence of fibres and other dusts within lung tissue is an important aspect of hazard assessment. Fibres that are persistent within lung tissue are believed to be more hazardous than less persistent fibres (Davis, 1994). The determination of lung burden in animal biopersistence studies requires that fibres are recovered without loss or damage (Warheit et al., 1991). If analysis is to be by electron microscopy, preparations must also contain a minimum of lung residue. A number of reagents have been used to recover fibres from lung tissue including sodium hypochlorite (bleach), potassium and sodium hydroxide, hydrochloric acid and hydrogen peroxide (Davis et al., 1986). Organic fibres such as para-aramid are reactive in these oxidising agents. Mineral dusts have also been recovered using enzymes, but not as a preparation suitable for scanning electron microscope (SEM) fibre counting (eg Nenadic and Crable, 1970). This communication describes an enzymatic tissue digestion procedure that we developed for the recovery of bleach-sensitive dusts from lung tissue. The technique was developed specifically for an investigation of the biopersistence of respirable para-aramid fibrils (Searl, in press a). The procedure described here was developed from a method used to digest tumour tissue (Evans and Cullen, 1984). The method proved unsuccessful for para-aramids, which partly disappear during tissue digestion, but may be useful for other fibres and dusts which are sensitive to oxidising agents.
