HISTOCHEMICAL OBSERVATIONS ON ENZYMATIC PROCESSES IN BONES AND TEETH
HISTOCHEMICAL OBSERVATIONS ON ENZYMATIC PROCESSES IN BONES AND TEETH
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DOI：
10.1111/j.1749-6632.1960.tb49972.x
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发表时间：
1960-01-01
影响因子：
5.2
通讯作者：
BURSTONE, MS
中科院分区：
文献类型：
--
作者：
BURSTONE, MS
酶被认为是控制骨骼和其他组织中化学反应速度的生物催化剂。由于钙化组织是包含几种不同类型的细胞和细胞间物质的非均一系统，所以通过常规生化程序对硬组织中的酶进行明确的研究存在一定的技术问题。另一方面，显微组织化学技术似乎为可视化和研究这些不同细胞成分的特定酶提供了极好的可能性。直到最近，碱性磷酸酶一直是组织化学研究中最重要的考虑因素。这主要是由于Gomori的原始磷酸酶技术对现代显微组织化学的推动。前述技术在钙化组织研究中的应用已在Bourne编辑的文本中进行了综述。2最近，一些与硬组织发育有关的水解酶的组织化学定位被报道。
Enzymes are considered to be the biological catalysts that control the rate of chemical reactions in bone as well as in other tissues. Definitive study of enzymes in hard tissues by means of routine biochemical procedures presents certain technical problems, since calcifying tissues are nonhomogenous systems containing: several different types of cells and intercellular substance. On the other hand, microscopic histochemical techniques appear to offer excellent possibilities for visualizing and studying specific enzymes of these various cellular components.Until recently alkaline phosphatase was given foremost consideration in histochemical studies. This is due primarily to the impetus given to modern microscopic histochemistry by the original phosphatase technique of Gomori? Application of the aforementioned technique in the study of calcifying tissues has been reviewed in the text edited by Bourne. 2 More recently histochemical localizations of a number of hydrolases associated with the development of hard tissues have been rep~ rted.~