Recipient range of IncP-7 conjugative plasmid pCAR2 from Pseudomonas putida HS01 is broader than from other Pseudomonas strains
Recipient range of IncP-7 conjugative plasmid pCAR2 from Pseudomonas putida HS01 is broader than from other Pseudomonas strains
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DOI：
10.1007/s10529-005-3892-1
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发表时间：
2005-12-01
影响因子：
2.7
通讯作者：
Nojiri, H
中科院分区：
文献类型：
--
作者：
Shintani, M;Habe, H;Nojiri, H
咔唑降解质粒 pCAR2 是从恶臭假单胞菌中分离出来的，其遗传结构与 pCAR1（IncP-7 原型质粒）相似。 pCAR2 与各种受体菌株的交配分析表明，它可以从 HS01 转移到假单胞菌受体：绿针假单胞菌、荧光假单胞菌、恶臭假单胞菌、食树脂假单胞菌和施氏假单胞菌。当不同的宿主被用作pCAR2的供体时，受体的范围发生了变化。来自菌株 HS01 的质粒范围比使用食树脂假单胞菌 CA10dm4 或恶臭假单胞菌 KT2440 的质粒范围更广。当 pCAR1 或 pCAR2 从相同的细胞背景转移时，缀合的范围和频率现在相似。定量 RT-PCR 分析表明，两个质粒上的 tra/trh 基因在每个供体菌株中的转录相似，这表明两个质粒的接合机制可能具有相似的功能，并且其他宿主因素正在影响受体范围和接合频率。
The carbazole-degradative plasmid pCAR2 was isolated from Pseudomonas putida and had a genetic structure similar to that of pCAR1, the IncP-7 archetype plasmid. Mating analyses of pCAR2 with various recipient strains showed that it could transfer from HS01 to Pseudomonas recipients: P. chlororaphis, P. fluorescens, P. putida, P. resinovorans and P. stutzeri. The range of recipients changed when different hosts were used as a donor of pCAR2. The range of the plasmid from strain HS01 was broader than that using P. resinovorans CA10dm4 or P. putida KT2440. When pCAR1 or pCAR2 was transferred from the same cell background, the range and frequency of conjugation were now similar. Quantitative RT-PCR analyses indicated that tra/trh genes on both plasmids were similarly transcribed in each donor strain suggesting that the conjugative machinery of both plasmids may function similarly, and that other host factors are affecting the recipient range and frequency of conjugation.