Evidence of newly generated neurons in the human olfactory bulb
Evidence of newly generated neurons in the human olfactory bulb
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DOI：
10.1016/j.devbrainres.2004.03.021
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发表时间：
2004-07-19
期刊：
影响因子：
--
通讯作者：
Parent, A
中科院分区：
文献类型：
--
作者：
Bédard, A;Parent, A
脑室下区（SVZ）是成人脑内神经干细胞的主要来源。在啮齿动物和非人灵长类动物中，SVZ中产生的许多神经母细胞沿着吻侧迁移流（RMS）成链迁移，以用新的颗粒和球周中间神经元填充嗅球（013）。为了了解这种现象是否存在于成年人的大脑中，我们应用单和双免疫染色程序的嗅球脑尸检后获得的正常成人受试者。双免疫荧光标记与共聚焦显微镜可视化细胞表达的标记物的增殖和未成熟的神经元状态，以及标记物，是特定的嗅觉interneurons。在人嗅球的颗粒层和肾小球层（GL）中检测到表达细胞周期蛋白[Ki-67，增殖细胞核抗原（PCNA）]的新生细胞;这些细胞共表达未成熟神经元状态的标志物，如Doublecortin（DCX），NeuroD和Nestin。许多分化细胞表达早期定型神经元的分子标记物[β-微管蛋白III类（TuJ 1）]，并且还对谷氨酸脱羧酶（GAD）（GABA能神经元的标记物）或酪氨酸羟化酶（TH）（多巴胺能神经元的标记物）具有免疫反应性。其他早期定型神经元表达钙结合蛋白calretinin（CR）或parvalbumin（PV）。这些结果为成人嗅觉系统神经发生的存在提供了有力的证据。尽管与啮齿类动物和非人类灵长类动物相比，人类的嗅球相对较小，但人类的嗅球似乎很密集。在整个生命过程中，通过表达各种化学表型的新颗粒和肾小球周围神经元。(C)2004 Elsevier B. V.保留所有权利。
The subventricular zone (SVZ) is known to be the major source of neural stem cells in the adult brain. In rodents and nonhuman primates, many neuroblasts generated in the SVZ migrate in chains along the rostral migratory stream (RMS) to populate the olfactory bulb (013) with new granular and periglomerular interneurons. In order to know if such a phenomenon exists in the adult human brain, we applied single and double immunostaining procedures to olfactory bulbs obtained following brain necropsy in normal adult human subjects. Double immunofluorescence labelling with a confocal microscope served to visualize cells that express markers of proliferation and immature neuronal state as well as markers that are specific to olfactory interneurons. Newborn cells that express cell cycle proteins [Ki-67, proliferating cell nuclear antigen (PCNA)] were detected in the granular and glomerular layers (GLs) of the human olfactory bulb; these cells coexpressed markers of immature neuronal state, such as Doublecortin (DCX), NeuroD and Nestin. Numerous differentiating cells expressed molecular markers of early committed neurons [beta-tubulin class III (TuJ1)] and were also immunoreactive for glutamic acid decarboxylase (GAD), a marker of GABAergic neurons, or tyrosine hydroxylase (TH), a marker of dopaminergic neurons. Other early committed neurons expressed the calcium-binding proteins calretinin (CR) or parvalbumin (PV). These results provide strong evidence for the existence of adult neurogenesis in the human olfactory system. Despite its relatively small size compared to that in rodents and nonhuman primates, the olfactory bulb in humans appears to be populated., throughout life, by new granular and periglomerular neurons that express a wide variety of chemical phenotypes. (C) 2004 Elsevier B.V. All rights reserved.