An evaluation of antioxidant, anti-inflammatory, and antinociceptive activities of essential oil from Curcuma longa. L.
An evaluation of antioxidant, anti-inflammatory, and antinociceptive activities of essential oil from Curcuma longa. L.
复制标题
DOI：
10.4103/0253-7613.84961
复制
发表时间：
2011-09
影响因子：
2.4
通讯作者：
Kuttan R
中科院分区：
文献类型：
--
作者：
Liju VB;Jeena K;Kuttan R
本研究旨在评估姜黄油的化学成分、体外和体内抗氧化潜力、抗炎和镇痛活性。通过气相色谱/质谱法对姜黄油进行化学分析。体外抗氧化活性通过六种不同的方法进行，体内抗氧化活性通过测量用佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）处理的巨噬细胞产生的超氧化物以及测定口服油一个月后的抗氧化水平来确定。使用角叉菜胶、葡聚糖和福尔马林在小鼠中研究了抗炎活性。通过使用乙酸诱导的小鼠扭体运动来评估抗伤害活性。姜黄精油的主要成分为ar-姜黄酮（61.79%）、curlone（12.48%）和ar-姜黄烯（6.11%）。姜黄油被发现具有体外抗氧化活性，清除超氧化物、羟基自由基和脂质过氧化的 IC50 分别为 135 μg/ml、200 μg/ml 和 400 μg/ml。 50 μg 姜黄精油的铁还原活性为 5 mM。研究发现腹腔内给予油可以抑制巨噬细胞引发的 PMA 诱导的超氧自由基。小鼠口服姜黄油1个月后，血液中超氧化物歧化酶、谷胱甘肽和谷胱甘肽还原酶水平以及肝脏中谷胱甘肽-S-转移酶和超氧化物歧化酶水平显着升高。姜黄油可显着减少角叉菜胶、葡聚糖诱发的急性炎症和福尔马林诱发的慢性炎症的爪子厚度。该药物在所有研究剂量下均产生显着的抗伤害活性（P < 0.001）。这些结果表明姜黄油具有潜在的健康益处，因为它可以清除自由基并产生显着的抗炎和镇痛活性。
This study was aimed to evaluate the chemical composition, antioxidant potential in vitro and in vivo, anti-inflammatory, and antinociceptive activity of turmeric oil. Chemical analysis of turmeric oil was done by gas chromatography/mass spectrometry. Antioxidant activities in vitro was done by six different methods and in vivo antioxidant activity was determined by measuring superoxide generation from macrophages treated with phorbol-12-myristate-13-acetate (PMA) as well as determining antioxidant level after feeding the oil orally for one month. Anti-inflammatory activity was studied in mice using carrageenan, dextran, and formalin. Antinociceptive activity was evaluated by using acetic acid-induced writhing movement in mice. The main constituent of essential oil of turmeric was found to be ar-turmerone (61.79%), curlone (12.48%), and ar-curcumene (6.11%). Turmeric oil was found to have in vitro antioxidant activity and IC50 for scavenging superoxides, hydroxyl radicals, and lipid peroxidation were 135 μg/ml, 200 μg/ml, and 400 μg/ml, respectively. The ferric-reducing activity for 50 μg of turmeric essential oil was found to be 5 mM. Intraperitoneal administration of oil was found to inhibit PMA-induced superoxide radicals elicited by macrophages. Oral administration of turmeric oil for one month to mice significantly increased superoxide dismutase, glutathione, and glutathione reductase enzyme levels in blood and glutathione-S-transferase and superoxide dismutase enzymes in liver. Turmeric oil showed significant reduction in paw thickness in carrageenan, dextran-induced acute inflammation, and formalin-induced chronic inflammation. The drug produced significant antinociceptive activity (P < 0.001) at all doses studied. These results demonstrated that turmeric oil has potential health benefits as it can scavenge the free radicals and produce significant anti-inflammatory and antinociceptive activities.