PROJECTION OF NONCHOLINERGIC BASAL FOREBRAIN NEURONS ENSHEATHED WITH PERINEURONAL NETS TO RAT MESOCORTEX
PROJECTION OF NONCHOLINERGIC BASAL FOREBRAIN NEURONS ENSHEATHED WITH PERINEURONAL NETS TO RAT MESOCORTEX
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DOI：
10.1016/0891-0618(94)90032-9
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发表时间：
1994-11-01
影响因子：
2.8
通讯作者：
BIGL, V
中科院分区：
文献类型：
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作者：
BRUCKNER, G;SCHUTZ, A;BIGL, V
一些作者使用逆行和顺行追踪技术记录了中隔海马系统中以及终止于嗅球和某些皮质区域的基底前脑投射中存在非胆碱能（GABA能）成分。另一方面，基底前脑还含有大量主要为小清蛋白阳性的神经元，这些神经元被聚阴离子蛋白聚糖的格子状基质包裹，形成所谓的功能尚不清楚的神经周围网。通过将氟金注射到大鼠中皮层区域的逆行追踪和紫藤凝集素（WFA）对神经周围网染色相结合，本研究描述了以基底前脑复合体前部（内侧隔核、布罗卡对角带核、大细胞视前核）中的神经周围网为特征的非胆碱能投射神经元的投射模式和分布。将示踪剂注射到扣带皮层后，标记的网络相关神经元分布在基底前脑复合体的头尾延伸内，但主要发现于布罗卡对角带的水平肢中。将示踪剂注射到压后皮质后，神经元周围神经网络的逆行标记在内侧隔膜中更为明显。胆碱乙酰转移酶免疫反应性（ChAT-ir）投射神经元在任何情况下都与神经元周围网无关。结果表明，基底前脑的大部分非胆碱能投射神经元被赋予了特殊的蛋白聚糖微环境，并形成了边缘系统中皮层成分的强大输入系统。
The existence of non-cholinergic (GABAergic) components in the septo-hippocampal system but also in basal forebrain projections terminating in the olfactory bulb and certain cortical areas has been documented by several authors using retrograde and anterograde tracing techniques. On the other hand, the basal forebrain also contains a high number of mainly parvalbumin-positive neurons ensheathed by a lattice-like matrix of polyanionic proteoglycans forming so-called perineuronal nets of as yet unknown function. By a combination of retrograde tracing using Fluoro-Gold injections into mesocortical areas of rats and staining of perineuronal nets by Wisteria floribunda agglutinin (WFA) the present study describes the projection pattern and distribution of non-cholinergic projection neurons characterized by perineuronal nets in the anterior parts of the basal forebrain complex (medial septal nucleus, nucleus of the diagonal band of Broca, magnocellular preoptic nucleus). After tracer injection into the cingulate cortex labelled net-associated neurons were distributed within the rostrocaudal extension of the basal forebrain complex but were predominantly found in the horizontal limb of the diagonal band of Broca. Retrograde labelling of neurons with perineuronal nets after tracer injection into the retrosplenial cortex was more pronounced in the medial septum. Choline acetyltransferase-immunoreactive (ChAT-ir) projection neurons were in no case associated with perineuronal nets. The results demonstrate that a large portion of the non-cholinergic projection neurons of the basal forebrain are endowed with a specialized microenvironment of proteoglycans and form a strong input system of mesocortical components of the limbic system.