Microsatellite variability within and among local landrace populations of tea, Camellia sinensis (L.) O.!Kuntze, in Kyoto, Japan
Microsatellite variability within and among local landrace populations of tea, Camellia sinensis (L.) O.!Kuntze, in Kyoto, Japan
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DOI：
10.1007/s10722-008-9311-4
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发表时间：
2008-11-01
影响因子：
2
通讯作者：
Oka, Kouzou
中科院分区：
文献类型：
--
作者：
Ohsako, Takanori;Ohgushi, Takafumi;Oka, Kouzou
茶树（Camellia sinensis（L.）O. Kuntze）位于日本京都南部，用6个微卫星标记进行了调查。地方品种群体的平均等位基因数为3.83 ~ 4.67，而现代品种和育种系的平均等位基因数为6.63。期望杂合度值平均在地方品种种群内的基因座范围从0.498到0.723。一个类似的变异水平，0.682，观察品种和育种系。每个群体的高固定指数值（0.177-0.417）与群体内的双亲近交一致。本地种群间的遗传分化极低，F-ST = 0.062，但AMOVA显示地方品种种群间存在显著分化。我们建议，这些人口共享一个共同的祖先基因库，并在一定程度上的人工选择，每个人口已由当地农民。相邻分析表明，居群间的遗传关系反映了居群的地理位置。这可能是由于附近农民更频繁地进行基因交换造成的。
Genetic variability within and among eight landrace populations of tea (Camellia sinensis (L.) O. Kuntze) located in southern Kyoto, Japan, was surveyed with six microsatellite markers. The average number of alleles per locus was 3.83 to 4.67 for landrace populations, whereas the corresponding value among modern cultivars and breeding lines was 6.63. Expected heterozygosity values averaged over loci within landrace populations ranged from 0.498 to 0.723. A similar level of variation, 0.682, was observed for cultivars and breeding lines. High fixation index values (0.177-0.417) for each population are consistent with biparental inbreeding within the population. Genetic differentiation among local populations was extremely low with F-ST = 0.062, although AMOVA revealed significant differentiation among landrace populations. We propose that these populations share a common ancestral gene pool and that some degree of artificial selection within each population has been performed by local farmers. Neighbor-joining analysis revealed that genetic relationships among populations reflect geographical location of populations. This might result from more frequent genetic exchange by nearby farmers.