A High Frequency of Beneficial Mutations Across Multiple Fitness Components in Saccharomyces cerevisiae
A High Frequency of Beneficial Mutations Across Multiple Fitness Components in Saccharomyces cerevisiae
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DOI：
10.1534/genetics.110.118307
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发表时间：
2010-08-01
期刊：
影响因子：
3.3
通讯作者：
Joseph, Sarah B.
中科院分区：
文献类型：
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作者：
Hall, David W.;Joseph, Sarah B.
突变累积实验被广泛用于估计影响适应度的自发突变的参数。在许多实验中，只测量了适合度的一个组成部分。在之前对二倍体酿酒酵母的研究中，我们测量了151个突变积累系的生长速度，以估计突变参数。我们发现，出人意料的高频率改变健康状况的突变是有益的。在这里，我们通过检查孢子形成效率、孢子活力和单倍体生长率来建立我们以前的工作，并发现这些适合度成分也显示出高频率的有益突变。我们还检查了突变-无累积(MA)线是否显示出累积突变之间的多效性的任何证据，并发现对于大多数人来说，没有任何证据。然而，对任何一个适应度成分具有零适应度(即致死性)的MA系确实显示出累积突变中的多效性的证据。我们还报告了基于适应度的每个组成部分对突变的其他参数的估计。
Mutation-accumulation experiments are widely used to estimate parameters of spontaneous mutations affecting fitness. In many experiments only one component of fitness is measured. In a previous study involving the diploid yeast Saccharomyces cerevisiae, we measured the growth rate of 151 mutation-accumulation lines to estimate parameters of mutation. We found that an unexpectedly high frequency of fitness-altering mutations was beneficial. Here, we build upon our previous work by examining sporulation efficiency, spore viability, and haploid growth rate and find that these components of fitness also show a high frequency of beneficial mutations. We also examine whether mutation-acycumulation (MA) lines show any evidence of pleiotropy among accumulated mutations and find that, for most, there is none. However, MA lines that have zero fitness (i.e., lethality) for any one fitness component do show evidence for pleiotropy among accumulated mutations. We also report estimates of other parameters of mutation based on each component of fitness.