GC/MS-based metabolomics reveals fatty acid biosynthesis and cholesterol metabolism in cell lines infected with influenza A virus
GC/MS-based metabolomics reveals fatty acid biosynthesis and cholesterol metabolism in cell lines infected with influenza A virus
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DOI：
10.1016/j.talanta.2010.09.019
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发表时间：
2010-11-15
期刊：
影响因子：
6.1
通讯作者：
Cai, Zongwei
中科院分区：
文献类型：
--
作者：
Lin, Shuhai;Liu, Ning;Cai, Zongwei
代谢组学是系统生物学的下游分支，是研究从细胞到生物体的代谢网络的重要手段。利用气相色谱-质谱仪(GC/MS)对甲型流感病毒感染的两种不同细胞系(A549和AGS)的代谢产物进行了分析，利用参考文库初步鉴定了A549和AGS细胞中的代谢物，并应用主成分分析(PCA)和聚类热图进行了解释和可视化，从而使代谢通量谱在病毒复制过程中能够区分脂肪酸的生物合成和胆固醇的代谢，并观察到脂肪酸周转的变化，代谢组学研究也揭示了A549和A549的不同反应与AGS细胞株对病毒感染的模式识别结果AGS细胞株可能对甲型流感病毒更敏感，而A549细胞株是分化较差的胃腺癌细胞株，而A549细胞系是相对分化的肺癌细胞株，推测病毒复制可能与细胞分化有关(C)2010爱思唯尔B V版权所有
Metabolomics is the downstream of systems biology and has drawn significant interest for studying the metabolic networks from cells to organisms To profile the metabolites in two different cell lines (A549 and AGS) infected with influenza A virus gas chromatography coupled with mass spectrometry (GC/MS) was employed Some differentiating metabolites in the cell lines were tentatively identified using reference library interpreted and visualized by applying principal components analysis (PCA) and cluster heat map Consequently metabolic flux profiling allowed the differentiation of fatty acid biosynthesis and cholesterol metabolism during viral replication in the cell lines The change in fatty acid turnover was also observed Metabolomics investigation also revealed the different responses between A549 and AGS cell lines to the virus infection From the pattern recognition results AGS cell line might be more susceptible to influenza A virus Regarding the fact that AGS is a poorly differentiated gastric adenocarcinoma cell line whereas A549 is a relatively differentiated lung tumor one it is speculated that viral replication might be associated with the cell differentiations (C) 2010 Elsevier B V All rights reserved