PATTERN REGULATION DURING REGENERATION OF LIMB STUMPS BEARING PARTIAL CIRCUMFERENCES OF FLANK SKIN IN THE NEWT, NOTOPHTHALMUS-VIRIDESCENS
PATTERN REGULATION DURING REGENERATION OF LIMB STUMPS BEARING PARTIAL CIRCUMFERENCES OF FLANK SKIN IN THE NEWT, NOTOPHTHALMUS-VIRIDESCENS
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DOI：
10.1002/jez.1402330111
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发表时间：
1985-01-01
影响因子：
--
通讯作者：
TANK, PW
中科院分区：
文献类型：
--
作者：
TANK, PW
以蝾螈为材料，研究了带部分侧腹皮肤的断肢的再生能力。viridescens。切除侧腹侧线背侧的皮肤，并用于替代上前肢的1/4、1/2或3/4皮肤。将移植物放置在相对于肢体轴线的前部、后部、背部或腹侧位置。移植物愈合30天后，通过移植物截肢，并允许再生60天。在85%的实验病例中，承载1/4周侧腹皮肤的肢体残端再生了完整的肢体。肢体1/2周皮肤替换后，57%再生完整肢体。当侧腹皮肤替代3/4的肢体皮肤时，29%的肢体再生完整。在早期的研究中，只有2%的实验肢体具有完整的侧翼皮肤周长，再生了完整的肢体（Tank，1984）。无论移植物大小如何，当侧腹皮肤移植物放置在肢体残端的腹侧位置时，观察到对图案形成的最大影响。结果进行了讨论的想法，肢体细胞之间的短程细胞相互作用是必不可少的模式形成肢体再生过程中。
The regenerative ability of limb stumps bearing partial circumferences of flank skin was tested in the newt, N. viridescens. Skin dorsal to the lateral line of the flank was removed and used to replace 1/4, 1/2 or 3/4 of the skin of the upper forelimb. Grafts were placed at anterior, posterior, dorsal, or ventral positions relative to the axes of the limb. After 30 days of graft healing the limbs were amputated through the grafts and were permitted to regenerate for 60 days. Limb stumps bearing 1/4 circumference of flank skin regenerated complete limbs in 85% of the experimental cases. After 1/2 circumference of limb skin was replaced, 57% regenerated complete limbs. When 3/4 of the limb skin was replaced by flank skin, 29% regenerated complete limbs. Only 2% of experimental limbs bearing a full circumference of flank skin regenerated complete limbs in an earlier study (Tank, 1984). The greatest effect on pattern formation was observed when flank skin grafts were placed at the ventral position of the limb stump regardless of graft size. Results are discussed in light of the idea that short-range cellular interactions between limb cells are essential for pattern formation during limb regeneration.