Rapid Quantitative Fluorescence Detection of Copper Ions with Disposable Microcapsule Arrays Utilizing Functional Nucleic Acid Strategy
Rapid Quantitative Fluorescence Detection of Copper Ions with Disposable Microcapsule Arrays Utilizing Functional Nucleic Acid Strategy
复制标题
利用功能核酸策略使用一次性微胶囊阵列快速定量荧光检测铜离子
DOI：
10.1038/s41598-018-36842-x
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发表时间：
2019-01-10
影响因子：
4.6
通讯作者：
Li, Zhihong
中科院分区：
文献类型：
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作者：
He, Enqi;Cai, Liangyuan;Li, Zhihong
在这项工作中，一个经济和易于使用的微胶囊阵列制造的冰印刷技术已实现超灵敏的荧光定量的铜离子采用功能核酸策略。采用冰印技术，检测试剂通过聚苯乙烯（PS）薄膜隔离和光聚合物密封，保证了功能性核酸反应的稳定和无污染环境。我们的微胶囊阵列在使用前以冷冻形式储存在−20 °C下具有长期稳定性（20天）。现场检测时，室温下用微量注射器将1 μL样品注入解冻的微胶囊中，20 min后用LED透照仪获得荧光结果。该方法的检测限可达100 nM（100 fmol/μL），具有较高的特异性。
In this work, an economical and easy-to-use microcapsule array fabricated by ice printing technique has been realized for ultrasensitive fluorescence quantification of copper ions employing functional nucleic acid strategy. With ice printing, the detection reagents are sealed by polystyrene (PS) film isolation and photopolymer, which guarantees a stable and contamination-free environment for functional nucleic acid reaction. Our microcapsule arrays have shown long-term stability (20 days) under −20 °C storage in frozen form before use. During the Cu2+on-site detection, 1 μL sample is simply injected into the thawy microcapsule by a microliter syringe under room temperature, and after 20 minutes the fluorescence result can be obtained by an LED transilluminator. This method can realize the detection limit to 100 nM (100 fmol/μL) with high specificity.