Morphology and electrophysiological properties of neurons projecting to the retrocerebral complex in the blow fly, Protophormia terraenovae
Morphology and electrophysiological properties of neurons projecting to the retrocerebral complex in the blow fly, Protophormia terraenovae
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DOI：
10.1007/s00441-004-0935-1
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发表时间：
2004-11-01
影响因子：
3.6
通讯作者：
Shiga, S
中科院分区：
文献类型：
--
作者：
Hamanaka, Y;Numata, H;Shiga, S
通过成年丽蝇 Protophormia terraenovae 的细胞内记录和染色，检查了脑间部 (PI) 和外侧部 (PL) 体细胞神经元的形态和电特性。根据体细胞的位置和纤维分布，鉴定出两种类型的PI神经元（PIa和PIb）和两种类型的PL神经元（PLa和PLb）。根据脑后复合体中的纤维分支模式，PIb 神经元进一步分为 PIb(1) 和 PIb(2) 两个亚组。 PIa神经元将轴突投射到对侧贲门体神经，而PLa和PLb神经元将轴突投射到同侧贲门神经。 PIb 神经元特征性地表现出对称形态，其体细胞沿中线分布。 PLb 神经元在食管下神经节中有一个大的分支区域。在大脑后复合体中，PIb(2) 和 PLA 神经元将纤维发送到软体。 PIa、PIb(1) 和 PLb 神经元不投射到软体，而是投射到其附近的心体-下脑复合体或内脏肌。 Pla、PIb 和 PLA 神经元表现出较长的尖峰持续时间（3-10 ms）。 PLb 神经元与抗 corazonin、FMRFamide 或 β-色素分散激素的抗血清发生免疫反应。这是第一份揭示成年果蝇 PI 和 PL 中体细胞单个神经元形态的报告。
Morphological and electrical properties of neurons with somata in the pars intercerebralis (PI) and pars lateralis (PL) were examined by intracellular recording and staining in the adult blow fly, Protophormia terraenovae. According to the location of somata and fiber distribution, two types of PI neurons (PIa and PIb) and two types of PL neurons (PLa and PLb) were identified. PIb neurons were further divided into two subgroups of PIb(1) and PIb(2) depending on fiber branching patterns in the retrocerebral complex. PIa neurons projected axons to the contralateral nervi corporis cardiaci, whereas PLa and PLb neurons projected axons to the ipsilateral nervi corporis cardiaci. PIb neurons characteristically showed symmetrical morphology with their somata along the midline. PLb neurons had a large branching area in the subesophageal ganglion. In the retrocerebral complex, PIb(2) and PLa neurons sent fibers into the corpus allatum. PIa, PIb(1) and PLb neurons projected not to the corpus allatum but to the corpus cardiacum-hypocerebral complex or visceral muscles in their vicinity. PIa, PIb and PLa neurons showed long spike durations (3-10 ms). PLb neurons were immunoreactive with antisera against corazonin, FMRFamide, or beta-pigment-dispersing hormone. This is the first report revealing the morphology of individual neurons with somata residing in PI and PL in the adult fly.