Subcellular immunolocalization of NMDA receptor subunit NR1, 2A, 2B in the rat vestibular periphery
Subcellular immunolocalization of NMDA receptor subunit NR1, 2A, 2B in the rat vestibular periphery
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DOI：
10.1016/s0006-8993(02)02419-8
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发表时间：
2002-05-10
期刊：
影响因子：
2.9
通讯作者：
Ishiyama, A
中科院分区：
文献类型：
--
作者：
Ishiyama, G;Lopez, I;Ishiyama, A
利用特异性抗肽抗体，在光镜和电镜水平上研究了NMDA谷氨酸受体NR1、NR2A和NR2B在大鼠前庭周围的免疫组织化学定位。ⅰ型前庭毛细胞的传入盏末和神经纤维均表现出较强的NR1、NR2A和NR2B免疫反应。电镜下，基底外侧I型毛细胞膜呈NR1免疫反应。11型毛细胞及其传入钮扣NR1、nr2a、NR2B无免疫反应。几乎所有的斯卡帕神经节神经元都有NR1免疫反应，但有一小部分NR2A无免疫反应。此外，较大的斯卡帕神经节神经元具有NR2B免疫反应，而较小的神经元无免疫反应。这些发现是功能性NMDA受体介导或调节传入兴奋性神经传递的有力证据，从T型而不是II型前庭毛细胞到初级传入神经。受体亚型可能是NR1/NR2A、NR1/NR2B和/或NR1/NR2A/NR2B的组合。(C) 2002 Elsevier Science BY。版权所有。
The immunohistochemical localization of the NMDA glutamate receptor subunits NR1, NR2A, and NR2B was investigated in the rat vestibular periphery at the light and electron microscopy level using specific antipeptide antibodies. The afferent calyceal terminals and nerve fibers innervating type I vestibular hair cells were strongly NR1, NR2A, and NR2B immunoreactive. Under electron microscopy, the basolateral type I hair cell membrane was NR1 immunoreactive. The type 11 hair cell and its afferent boutons were NR1, NR2A. and NR2B non-immunoreactive. Nearly all of Scarpa's ganglion neurons were NR1 immunoreactive, but there was a subset of NR2A non-immunoreactive neurons. Additionally, the larger sized Scarpa's ganglia neurons were NR2B immunoreactive, while the Smaller neurons were non-immunoreactive. These findings are strong evidence for functional NMDA receptor mediation or modulation of afferent excitatory neurotransmission from type T but not type II vestibular hair cells to the primary afferent nerve. The receptor subtype(s) may be a combination of NR1/NR2A, NR1/NR2B, and/or NR1/NR2A/NR2B. (C) 2002 Elsevier Science BY. All rights reserved.