Foxd1-dependent induction of a temporal retinal character is required for visual function.
Foxd1-dependent induction of a temporal retinal character is required for visual function.
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DOI：
10.1242/dev.200938
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发表时间：
2022-12-15
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--
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胚胎发育过程中视网膜的适当图案被认为是建立介导特定视觉特征感知的空间局部特化的基础。例如，在斑马鱼中，与高敏锐度视力（HAA）相关的区域被认为存在于腹颞视网膜中。在这里，我们发现转录因子 Rx3 与成纤维细胞生长因子和 Hedgehog 信号的相互作用启动并限制了 Foxd1 表达到预期颞视网膜，从而启动视网膜的鼻颞区域化。 Foxd1 的缺失会导致颞部视网膜特征的丧失和鼻部视网膜特征的扩展，从而导致 HAA 的缺失。根据视动和视运动反应测定的评估，这些结构缺陷与严重的视觉缺陷相关。相反，在相反的情况下，视动反应不受影响，在这种情况下，鼻视网膜特征以扩大的时间特征为代价而丢失。我们的研究表明，在早期视网膜发育过程中颞部视网膜特征的建立是HAA规范所必需的，并表明颞部视网膜在控制特定视觉功能中发挥着重要作用。摘要：这项研究提供了眼睛图案和功能不同的视网膜区域建立之间的机制联系，并揭示了颞部视网膜优先控制视觉功能的特定方面。
Appropriate patterning of the retina during embryonic development is assumed to underlie the establishment of spatially localised specialisations that mediate the perception of specific visual features. For example, in zebrafish, an area involved in high acuity vision (HAA) is thought to be present in the ventro-temporal retina. Here, we show that the interplay of the transcription factor Rx3 with Fibroblast Growth Factor and Hedgehog signals initiates and restricts foxd1 expression to the prospective temporal retina, initiating naso-temporal regionalisation of the retina. Abrogation of Foxd1 results in the loss of temporal and expansion of nasal retinal character, and consequent absence of the HAA. These structural defects correlate with severe visual defects, as assessed in optokinetic and optomotor response assays. In contrast, optokinetic responses are unaffected in the opposite condition, in which nasal retinal character is lost at the expense of expanded temporal character. Our study indicates that the establishment of temporal retinal character during early retinal development is required for the specification of the HAA, and suggests a prominent role of the temporal retina in controlling specific visual functions. Summary: This study provides a mechanistic link between eye patterning and the establishment of functionally distinct retinal regions, and reveals that the temporal retina preferentially controls specific aspects of visual function.
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