Chemical composition and antimicrobial activity of the extracts of Kefe cumin (Laser trilobum L.) fruits from different regions
Chemical composition and antimicrobial activity of the extracts of Kefe cumin (Laser trilobum L.) fruits from different regions
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DOI：
10.3109/09637480801993938
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发表时间：
2009-01-01
影响因子：
3.9
通讯作者：
Ozcan, Mehmet Musa
中科院分区：
文献类型：
--
作者：
Parlatan, Ahmet;Saricoban, Cemalettin;Ozcan, Mehmet Musa
某些化合物（纯化学品、精油、提取物等）的结构特性（使用气相色谱分析：气相色谱和气相色谱-质谱）采用琼脂稀释法和琼脂扩散法对克非孜然（Laser trilobum L.）从不同地区（德国、印度和土耳其）获得。经气相色谱-质谱联用分析，确定其主要成分为柠檬烯（41.03-72.24%）和紫苏醛（4.23-32.75%）。采用电感耦合等离子体原子发射光谱仪测定了果实中的矿物质含量。经测定，其果实中含有丰富的钾、钙、磷、镁、钠、砷、铝等矿物质。在从土耳其获得的样品中发现最高的钠含量为9，261.28 mg/kg，但是来自德国和印度的样品的钾含量高于从土耳其获得的Kefe孜然果实。结果表明，该果实提取物对金黄色葡萄球菌、普通变形杆菌（FMC 1）、奇异变形杆菌、蜡样芽孢杆菌（FMC 19）、嗜水气单胞菌（Aeromonaserapa（ATCC 7965），粪肠球菌（ATCC 15753）、肺炎克雷伯菌（FMC 5）、鼠伤寒沙门氏菌、产气肠杆菌（CCM 2531）和大肠杆菌（ATCC 8739），而没有观察到精油的显著抗微生物作用。
Structural properties (using gas chromatographic analyses: gas chromatography and gas chromatography-mass spectrometry) of some compounds (pure chemicals, essential oils, extracts, etc.) and systematic investigation of antimicrobial activities (with agar dilution and agar diffusion disc methods) were determined in the fruits of Kefe cumin (Laser trilobum L.) obtained from different regions (Germany, India, and Turkey). The main components were established as limonene (41.03-72.24%) and perillaldehyde (4.23-32.75%) in the essential oils determined by gas chromatography-mass spectrometry. Mineral contents in the fruits were determined by inductively coupled plasma atomic emission spectrometer. It was determined that the fruits were rich in potassium, calcium, phosphorus, magnesium, sodium, arsenic and aluminium minerals. The highest sodium content was found to be 9,261.28mg/kg in the samples obtained from Turkey, but the potassium content of the samples from Germany and India was higher than that of Kefe cumin fruits obtained from Turkey. The fruit extracts had a significant antimicrobial effect on pathogen bacteria such as Staphylococcus aureus, Proteus vulgaris (FMC 1), Proteus mirabilis, Bacillus cereus (FMC 19), Aeromonas hydrophila (ATCC 7965), Enterococcus faecalis (ATCC 15753), Klebsiella pneumoniae (FMC 5), Salmonella typhimurium, Enterobacter aerogenes (CCM 2531) and Escherichia coli (ATCC 8739), whereas no significant antimicrobial effect of the essential oils was observed.