Mechanisms controlling function in the clasp and sling regions of porcine lower oesophageal sphincter
Mechanisms controlling function in the clasp and sling regions of porcine lower oesophageal sphincter
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DOI：
10.1002/bjs.5831
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发表时间：
2007-11-01
影响因子：
9.6
通讯作者：
Clave, P.
中科院分区：
文献类型：
--
作者：
Farre, R.;Auli, M.;Clave, P.
背景资料：下食管括约肌（LOS）卡环和吊索肌肉之间的功能差异的表征可能有助于更具体的药理学和手术方法的发展，用于治疗运动障碍。方法：在器官浴中研究了25只成年猪的圆形LOS条，以比较卡环和吊索纤维的生理学。7.59（0.89）vs 4.72（0.67）g; P = 0.017）。LOS张力更依赖于细胞外钙在扣带和胆碱能肠运动神经元（EMN）在吊索条的活动。电场刺激（EFS，3 Hz）引起的EMN最大舒张幅度在卡环条中更大（平均值（s.e.m.）74.5（2.3）对58.1（2.2）%的音调; P < 0.001）。通过使用10 μ mol/l 1H-[1，2，4]恶二唑-[4，3-alpha]喹喔啉-1-酮（ODQ）进行氮能阻断，扣紧纤维中的EFS诱导的松弛被减少，吊带纤维中的EFS诱导的松弛被完全阻断。EFS胆碱能反应的幅度在吊索纤维中显著更大。在扣带区域，由EMN与100 μ mol/L尼古丁刺激引起的松弛减少ODQ。在吊索纤维，尼古丁引起的放松休息和胆碱能收缩后ODQ。结论：扣和吊索纤维的猪LOS显示出显着的内在功能差异。这应该被考虑时，开发更具体的方法来人类LOS动力障碍。
Background: Characterization of functional differences between lower oesophageal sphincter (LOS) clasp and sling muscles might aid the development of more specific pharmacological and surgical approaches for the treatment of motility disorders.Methods: Circular LOS strips from 25 adult pigs were studied in organ baths to compare the physiology of clasp and sling fibres.Results: Sling strips developed greater tone than clasp fibres (mean(s.e.m.) 7.59(0.89) versus 4.72(0.67) g; P = 0.017). LOS tone was more dependent on extracellular calcium in clasp strips and on the activity of cholinergic enteric motor neurones (EMNs) in sling strips. The amplitude of maximal relaxation caused by electrical field stimulation (EFS, 3Hz) of EMNs was greater in clasp strips (mean(s.e.m.) 74.5(2.3) versus 58.1(2.2) per cent of tone; P < 0.001). EFS-induced relaxation was reduced in clasp fibres and fully blocked in sling fibres by nitrergic blockade with 10 mu mol/l 1H-[1,2,4]oxadiazole-[4,3-alpha]quinoxalin-1-one (ODQ). The amplitude of EFS cholinergic responses was significantly greater in sling fibres. In the clasp region, relaxation caused by stimulation of EMNs with 100 mu mol/l nicotine was reduced by ODQ. In sling fibres, nicotine induced relaxation at rest and cholinergic contraction following ODQ.Conclusion: Clasp and sling fibres of the porcine LOS show marked intrinsic functional differences. This should be considered when developing more specific approaches to human LOS motility disorders.