Characterization and mapping of the Xiphophorus maculatus (Teleostei: Poeciliidae) RPS15 gene.
Characterization and mapping of the Xiphophorus maculatus (Teleostei: Poeciliidae) RPS15 gene.
复制标题
剑尾鱼（Teleostei：Poeciliidae）RPS15 基因的特征和定位。
DOI：
10.1159/000134465
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Nairn,RS
中科院分区：
文献类型：
--
作者：
Walter,RB;Obermoeller,RD;Moore,DD;Lacson,JM;DellaColetta,L;McEntire,BB;Morizot,DC;Nairn,RS
We have determined the nucleotide sequence and gene map location of theXiphophorus mαculαtushomologue of RPS 15 (ribosomal protein S15, alias RIG). TheXiphophorusRPS15 cDNA encodes 145 amino acids, which show 94 % identity compared to deduced mammalian and avian RPS15 amino acid sequences. At the nucleotide level, 84% sequence identity is maintained between the fish and human gene, while homologous amphibian and avian sequences show about 80% nucleotide identity compared to theXiphophorussequence. Nucleotide identity substantially decreases when the fish gene is compared toArabidopsisS15 (64%) and yeast S21 (55%) genes. Genetic linkage analysis of an RPS 15 restriction fragment length polymorphism in backcross hybrids generated from the cross X.hellerí ×(X. maculatusJp 163 B × Xhelleri) demonstrated linkage ofXiphophorusRPS 15 to the EGFR, UMPK and YES loci inXiphophorusLinkage Group VI.
