6-AZAURACIL INHIBITION OF GTP BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE
6-AZAURACIL INHIBITION OF GTP BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE
复制标题
DOI：
10.1007/bf00351735
复制
发表时间：
1992-07-01
期刊：
影响因子：
2.5
通讯作者：
LACROUTE, F
中科院分区：
文献类型：
--
作者：
EXINGER, F;LACROUTE, F
在生长培养基中添加 6-氮杂尿嘧啶会导致酿酒酵母核苷酸库中 GTP 水平大幅降低。体外实验表明 6-azaUMP 强烈抑制 IMP 脱氢酶活性，这解释了上述效果。 PPR2 突变体之前的特征是与野生型相比对 6-氮尿嘧啶的敏感性增加，特别容易受到 GTP 池降低的影响，并且当向培养基中添加鸟嘌呤时，能够在 6-氮尿嘧啶存在下生长。
The addition of 6-azauracil to the growth medium causes a strong reduction of the GTP level in the nucleotide pool of Saccharomyces cerevisiae. In-vitro experiments show a strong inhibition of IMP dehydrogenase activity by 6-azaUMP explaining the preceeding effect. PPR2 mutants, previously characterized by an increased sensitivity to 6-azauracil compared to the wild-type, are specifically susceptible to the lowering of the GTP pool, and are able to grow in presence of 6-azauracil when guanine is added to the medium.