Molecular characterization and expression analysis of interferon-gamma in the large yellow croaker Larimichthys crocea
Molecular characterization and expression analysis of interferon-gamma in the large yellow croaker Larimichthys crocea
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DOI：
10.1016/j.fsi.2015.07.008
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发表时间：
2015-10-01
影响因子：
4.7
通讯作者：
Wu, Chang-Wen
中科院分区：
文献类型：
--
作者：
Chen, Ruan-Ni;Su, Yong-Quan;Wu, Chang-Wen
大黄鱼（Larimichthys crocea）是中国重要的海水养殖鱼类，其养殖领域的两大致病菌是哈氏弧菌（Vibrio harveyi）和刺激性隐核菌（crypcaryon irritans）。干扰素- γ (ifn - γ)作为炎症细胞因子在调节先天和细胞介导的免疫反应中发挥重要作用。在这项研究中，我们从大黄鱼（LcIFN-gamma）中获得了ifn - γ的核苷酸序列。根据18个可用ifn - γ基因的序列构建了系统发育关系树。分别用V. harveyi和C. irritans攻毒后，在10个不同组织中进行了表达分析。实时PCR分析显示，lcifn - γ在健康个体中广泛表达。注射V. harveyi后，10个组织在第一天（1 d） ifn - γ表达较高；在刺激c感染1 d后，只有脾脏、肌肉、肠道、心脏和皮肤表达较高。分别于0 h、6 h、1 d、2 d、3 d、4 d、5 d和7 d采集主要免疫组织（皮肤、鳃、头部肾和脾脏）和解毒组织（肝脏），了解刺激c感染后lcifn - γ的表达趋势。lcifn - γ在皮肤和鳃（主要免疫器官）中的表达与刺激C的生命周期有明显的相关关系。(C) 2015 Elsevier Ltd.版权所有。
The large yellow croaker Larimichthys crocea is an important mariculture fish species in China, and the bacterium Vibrio harveyi (V. harveyi) and the ciliate protozoan Cryptocaryon irritans (C. irritans) are the two major pathogens in its aquaculture sector. Interferon-gamma (IFN-gamma) plays important roles in regulating both innate and cell mediated immune responses as an inflammatory cytokine. In this study, we obtained the nucleotide sequence of IFN-gamma from the large yellow croaker (LcIFN-gamma). The phylogenetic relationship tree of 18 available IFN-gamma genes was constructed based on their sequences. Expression analyses in 10 various tissues were conducted after the croaker challenged with V. harveyi and C. irritans, respectively. Real time PCR analysis showed that the expression of LcIFN-gamma was observed broadly in health individuals. After injected with V. harveyi, the 10 tissues had a higher expression of IFN-gamma at the first day (1 d); only spleen, muscle, intestine, heart and skin had higher expressions after infected with C. irritans at 1 d. Major immune tissues (skin, gill, head kidney and spleen) and detoxification tissue (liver) were sampled at 0 h, 6 h, 1 d, 2 d, 3 d, 4 d, 5 d and 7 d to understand the expression trends of LcIFN-gamma after challenged with C. irritans. The expressions of LcIFN-gamma in skin and gill (the primary immune organs) showed a clear correlative relationship with the life cycle of C irritans. (C) 2015 Elsevier Ltd. All rights reserved.