Superantigen genes encoded by the egc cluster and SaPIbov are predominant among Staphylococcus aureus isolates from cows, goats, sheep, rabbits and poultry.
Superantigen genes encoded by the egc cluster and SaPIbov are predominant among Staphylococcus aureus isolates from cows, goats, sheep, rabbits and poultry.
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由egc簇和SaPIbov编码的超抗原基因在牛、山羊、绵羊、兔和家禽的金黄色葡萄球菌分离物中占主导地位。
DOI：
10.1099/jmm.0.45863-0
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Smyth,CyrilJ
中科院分区：
文献类型：
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作者：
Smyth,DavidaS;Hartigan,PatrickJ;Meaney,WilliamJ;Fitzgerald,JRoss;Deobald,ClaudiaF;Bohach,GregoryA;Smyth,CyrilJ
近年来，一些新的葡萄球菌肠毒素（SE）已被描述，目前在很大程度上未知的发生频率和在人类或动物疾病中的作用。用多重PCR和单PCR方法对191株来自牛（99株）、山羊（39株）、绵羊（23株）、兔（15株）、鸡（15株）和猫（1株）的金黄色葡萄球菌分离株进行了SE基因sea-see、sea-seo和seq以及编码葡萄球菌中毒性休克综合征毒素-1（STT）基因的筛选。110个分离株检测结果为阳性，至少有一个这16个超抗原（SAg）编码基因。来自不同动物的分离株之间的一些SAg基因的频率有统计学显著差异。没有菌株具有这两个基因。51株分离株中存在ec基因，8株存在esd基因，1株存在esb基因。在4株分离株中发现了该基因，在1株分离株中同时发现了该基因和seq基因。最常见的基因组合是47个分离物中的egc簇，携带seg、sei、sem、senandseogenes，44个分离物中的ec、sei和tst基因组合是典型的SaPIbov毒力岛，11个分离物中的egc簇缺少seg基因，9个分离物中的sed和sej基因，7个分离物中的ecandtst基因缺少sei基因。SAg基因阳性的绵羊或山羊分离株中，与牛分离株相比，这两个基因在一起的频率较高，而egc基因簇的频率较低，具有统计学意义。36牛分离株，有丝分裂的人T淋巴细胞，4个是阴性的16个SAg基因测试，而另外14个给临界结果，在有丝分裂试验，其中12个SAg基因阴性。29株缺乏所有的SAg基因没有诱导T细胞增殖。本研究表明，新的SE基因seg、sei、sel、sem、senand seo以及这些基因在动物宿主金黄色葡萄球菌中占优势。促有丝分裂试验表明，牛分离株中可能存在其他未表征的SAgs。
In recent years several new staphylococcal enterotoxins (SEs) have been described, which currently have largely unknown frequencies of occurrence and roles in human or animal disease. One hundred and ninety-oneStaphylococcus aureusisolates from cows (99), goats (39), sheep (23), rabbits (15), chickens (15) and a cat (1) were screened for SE genessea–see,seg–seoandseqand for thetstgene encoding staphylococcal toxic shock syndrome toxin-1 using multiplex PCRs and individual PCRs for thesebandsekgenes. One hundred and ten isolates tested positive for at least one of these 16 superantigen (SAg)-encoding genes. There were statistically significant differences in the frequencies of some of these SAg genes between isolates from different animals. No strain possessed either theseaorseegene. Thesecgene was present in 51 isolates, thesedgene in eight and thesebgene in one. Thesehgene was found in four strains and thesekandseqgenes together in one isolate. The most common combinations of genes were theegccluster, bearing theseg,sei,sem,senandseogenes, in 47 isolates, thesec,selandtstgene combination typical of the SaPIbov pathogenicity island in 44 isolates, theegccluster lacking theseggene in 11 isolates, thesedandsejgenes in nine isolates, and thesecandtstgenes without theselgene in seven isolates. The higher frequencies of thesecandtstgenes together and the lower frequencies of theegcgene cluster among the SAg gene-positive sheep or goat isolates compared to bovine isolates were statistically significant. Of 36 bovine isolates that were mitogenic for human T lymphocytes, four were negative for the 16 SAg genes tested for, while a further 14 gave borderline results in the mitogenicity assay, 12 of which were SAg gene-negative. Twenty-nine strains lacking all the SAg genes did not induce T-cell proliferation. This survey indicates that novel SE genesseg,sei,sel,sem,senandseoalong with thesecandtstgenes predominate inS. aureusfrom animal hosts. The mitogenicity assays indicate that further uncharacterized SAgs may be present in bovine isolates.