Isolation and characterization of new fission yeast cytokinesis mutants.
Isolation and characterization of new fission yeast cytokinesis mutants.
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新裂殖酵母胞质分裂突变体的分离和表征。
DOI：
10.1093/genetics/149.3.1265
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发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Gould,KL
中科院分区：
文献类型：
--
作者：
Balasubramanian,MK;McCollum,D;Chang,L;Wong,KC;Naqvi,NI;He,X;Sazer,S;Gould,KL
粟酒裂殖酵母是研究胞质分裂的极好生物，因为它使用F-肌动蛋白收缩环进行中间分裂。为了加强我们对细胞分裂过程的理解，进行了一次大的遗传筛选，其中鉴定了17个胞质分裂所必需的遗传位点，其中5个是新的。识别三个基因，rng 3+，rng 4+，和rng 5+的突变体，在组织肌动蛋白收缩环有缺陷。还鉴定了四种隔膜沉积缺陷突变体，隔膜起始缺陷（SID）1、SID 2、SID 3和SID 4。遗传分析表明，sid突变体与之前描述的分隔突变体cdc 7 -24、cdc 11 -123和cdc 14 -118表现出强烈的负相互作用。rng 5+、rng 2+和rng 3+基因被克隆并显示分别编码Myo 2 p（一种肌球蛋白重链）（一种与芽殖酵母Dbf 2 p相关的蛋白激酶）和Spg 1 p（一种GTP结合蛋白，是GTP酶的ras超家族的成员）。Spg 1 p从细胞周期的任何点促进隔膜形成的能力取决于Sid 4p的活性。此外，我们的特点还没有被描述以前在胞质分裂突变体，即未能重组肌动蛋白补丁的中间区域的细胞准备隔膜形成的表型。
Schizosaccharomyces pombe is an excellent organism in which to study cytokinesis as it divides by medial fission using an F-actin contractile ring. To enhance our understanding of the cell division process, a large genetic screen was carried out in which 17 genetic loci essential for cytokinesis were identified, 5 of which are novel. Mutants identifying three genes, rng3+, rng4+, and rng5+, were defective in organizing an actin contractile ring. Four mutants defective in septum deposition, septum initiation defective (sid)1, sid2, sid3, and sid4, were also identified and characterized. Genetic analyses revealed that the sid mutants display strong negative interactions with the previously described septation mutants cdc7-24, cdc11-123, and cdc14-118. The rng5+, sid2+, and sid3+genes were cloned and shown to encode Myo2p (a myosin heavy chain), a protein kinase related to budding yeast Dbf2p, and Spg1p, a GTP binding protein that is a member of the ras superfamily of GTPases, respectively. The ability of Spg1p to promote septum formation from any point in the cell cycle depends on the activity of Sid4p. In addition, we have characterized a phenotype that has not been described previously in cytokinesis mutants, namely the failure to reorganize actin patches to the medial region of the cell in preparation for septum formation.