Hypoglycemic effects of polysaccharides from Gomphidiaceae rutilus fruiting bodies and their mechanisms
Hypoglycemic effects of polysaccharides from Gomphidiaceae rutilus fruiting bodies and their mechanisms
复制标题
红花子实体多糖的降血糖作用及其机制
DOI：
10.1039/c9fo02283j
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发表时间：
2020-01-01
期刊：
影响因子：
6.1
通讯作者：
Fan, Yuying
中科院分区：
文献类型：
--
作者：
Yang, Siwen;Qu, Yunhe;Fan, Yuying
胰岛素抵抗是2型糖尿病、脂肪肝和肥胖的主要原因。我们前期的研究发现蘑菇多糖在体外能改善胰岛素抵抗，但其机制尚不清楚。因此，我们研究了红毛菇子实体多糖的降血糖作用及其机制。在高葡萄糖和脂肪酸处理的肝细胞中，来自Gomphidiaceae rutilus子实体的总多糖（AGRP）和中性多糖（AGRP-N）级分均以自噬依赖的方式增强胰岛素刺激的葡萄糖摄取，但酸性多糖（AGRP-A）级分不增强。此外，我们阐明了口服多糖对ob/ob小鼠的降血糖作用。AGRP和AGRP-N降低血糖并改善胰岛素敏感性。它们抑制肝脏脂质沉积，不仅通过激活AMPK以增加自噬，而且通过增加PPAR α和CPT-1a的表达以增强脂解。本研究结果为红毛菇多糖作为降血糖保健食品的开发提供了依据。
Insulin resistance is the main cause of type 2 diabetes, fatty liver and obesity. Our previous study found that mushroom polysaccharides improved insulin resistance in vitro, but the underlying mechanisms were still unknown. Thus, we investigate the hypoglycemic effects of polysaccharides from Gomphidiaceae rutilus fruiting bodies and their mechanisms. The total polysaccharides (AGRP) from Gomphidiaceae rutilus fruiting bodies and the neutral polysaccharide (AGRP-N) fraction both enhance insulin-stimulated glucose uptake in an autophagy-dependent manner in high glucose and fatty acid-treated hepatic cells, but not the acidic polysaccharide (AGRP-A) fraction. Further, we elucidate the oral hypoglycemic effects of polysaccharides on ob/ob mice. AGRP and AGRP-N lower blood glucose and improve insulin sensitivity. They inhibit liver lipid deposition, not only by activating AMPK to increase autophagy but also by increasing the expressions of PPAR alpha and CPT-1a to enhance lipolysis. Our results provide a basis for the development of polysaccharides from Gomphidiaceae rutilus as a hypoglycemic healthy food.