Standardized bacteriologic techniques for the characterization of Mycoplasma species1, 2
Standardized bacteriologic techniques for the characterization of Mycoplasma species1, 2
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用于表征支原体种类的标准化细菌学技术1, 2
DOI：
10.1099/00207713-20-1-35
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发表时间：
1970
影响因子：
2.8
通讯作者：
J. Faber
中科院分区：
文献类型：
--
作者：
Barbara B. Aluotto;R. Wittler;Caroline Williams;J. Faber
摘要 研究了适用于支原体物种表征和分化的细菌学方法。经过适当的修改和标准化后，这些方法在包含超过 22 个物种的 53 种支原体菌株上进行了测试。最适合表征和区分目的的测试是：通过氧化或发酵分解葡萄糖，精氨酸或尿素水解，四唑、亚碲酸盐和亚甲蓝还原，磷酸酶活性，薄膜和斑点的产生，明胶水解，酪蛋白和凝固血清的消化，对奥普托钦的敏感性，以及绵羊红细胞的溶血。
ABSTRACT Bacteriological methods applicable to the characterization and differentiation of Mycoplasma species were investigated. After appropriate modification and standardization, these methods were tested on 53 Mycoplasma strains comprising more than 22 species. The tests found most suitable for purposes of characterization and differentiation were: breakdown of glucose by oxidation or fermentation, hydrolysis of arginine or urea, reduction of tetrazolium, tellurite, and methylene blue, phosphatase activity, production of film and spots, hydrolysis of gelatin, digestion of casein and coagulated serum, sensitivity to optochin, and hemolysis of sheep erythrocytes.