A trial for a DNA diagnosis of Plasmodium vivax malaria recently reemerging in the Republic of Korea using microtiter plate hybridization assay
A trial for a DNA diagnosis of Plasmodium vivax malaria recently reemerging in the Republic of Korea using microtiter plate hybridization assay
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DOI：
10.4269/ajtmh.2000.63.80
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发表时间：
2000-07-01
影响因子：
3.3
通讯作者：
Wataya, Y
中科院分区：
文献类型：
--
作者：
Chai, JY;Park, YK;Wataya, Y
聚合酶链反应为基础的微量滴定板杂交（PCR-MPH）分析用于间日疟原虫疟疾，最近在大韩民国重新出现的DNA诊断。受试者为18名寄生虫证实的患者和5名健康对照。在标准疗程后，从4名患者中采集随访血液样本。聚合酶链反应和电泳显示所有患者的血液在138个碱基对区域有一条突出的条带，而对照组和治疗后的患者则没有。PCR产物与各种疟疾物种的18 S rRNA的已知物种特异性探针杂交，在所有患者中显示出对间日疟原虫特异性探针的强阳性反应（在405 nm处吸光度1.30-1.90）。吸光度与血寄生虫血症程度呈正相关，但有临界意义。对韩国间日疟原虫的探针区域进行测序，结果显示与典型间日疟原虫没有显著差异。结果表明，PCR-MPH是一种非常有用的技术，用于韩国间日疟的DNA诊断。
The polymerase chain reaction-based microtiter plate hybridization (PCR-MPH) assay was utilized for a DNA diagnosis of Plasmodium vivax malaria, which has recently reemerged in the Republic of Korea. The subjects were 18 parasite-proven patients and 5 healthy controls. Follow-up blood samples were collected from 4 patients after a standard course of treatment. Polymerase chain reaction and electrophoresis of all the patients' blood showed a prominent band at the 138 base pair area, but not in the controls or after treating the patients. Hybridization of the PCR produces with known species-specific probes of the 18S rRNA of various malaria species revealed strong positive reactions against the Plasmodium vivax-specific probe (absorbance 1.30-1.90 at 405 nm) in ail of the patients. The absorbance was positively correlated with the degree of blood parasitemia, but with a borderline significance. Sequencing of the probe region of the Korean P. vivax revealed no significant variations from the typical P. vivax. The results show that the PCR-MPH is a highly useful technique for the DNA diagnosis of Korean vivax malaria.