Cytokine analysis by immunoaffinity capillary electrophoresis.
Cytokine analysis by immunoaffinity capillary electrophoresis.
复制标题
通过免疫亲和毛细管电泳进行细胞因子分析。
DOI：
10.1007/978-1-62703-029-8_23
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kalish,Heather
中科院分区：
文献类型：
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作者：
Mendonca,Mark;Kalish,Heather
免疫亲和毛细管电泳（ICE）是一种用于检测和定量复杂生物流体中目标蛋白的有力工具。目标分析物被捕获并与固定在毛细管壁上的抗体结合，用荧光染料原位标记，洗脱并在电泳分离后使用激光诱导荧光在线检测。在这里，我们说明了如何构建一个免疫亲和毛细管，并利用它来运行ICE，以捕获和定量目标细胞因子和趋化因子从临床样本。
Immunoaffinity capillary electrophoresis (ICE) is a powerful tool used to detect and quantify target proteins of interest in complex biological fluids. The target analyte is captured and bound to antibodies immobilized onto the wall of a capillary, labeled in situ with a fluorescent dye, eluted and detected online using laser-induced fluorescence following electrophoretic separation. Here, we illustrate how to construct an immunoaffinity capillary and utilize it to run ICE in order to capture and quantify target cytokines and chemokines from a clinical sample.