DISTRIBUTION OF FLUORESCENCE IN HUMAN CATARACTOUS LENS
DISTRIBUTION OF FLUORESCENCE IN HUMAN CATARACTOUS LENS
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DOI：
10.1159/000264754
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发表时间：
1975-01-01
影响因子：
2.1
通讯作者：
AUGUSTEYN, RC
中科院分区：
文献类型：
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作者：
AUGUSTEYN, RC
正常晶状体和白内障晶状体含有一种高荧光的黄色蛋白质，该蛋白质不溶于水，但可溶于含有0.01M硫醇的7M尿素。这种蛋白质的含量随着白内障的发展而增加，这伴随着晶状体中水溶性蛋白质含量的下降。在7M尿素和巯基乙醇存在下，蛋白质的相对分子质量超过8万。它似乎是通过二硫键以外的共价键交联的可溶性晶体亚单位的衍生物。
Normal and cataractous lenses contain a highly fluorescent yellow protein which is insoluble in water but soluble in 7 m urea containing 0.01 m mercaptoethanol. The amount of this protein increases with the development of cataracts and this is accompanied by a decrease in the water-soluble protein content of the lens. The protein has a molecular weight of over 80,000 in the presence of 7 m urea and mercaptoethanol. It appears to be a derivative of the sub-units of the soluble crystalline cross-linked through covalent bonds other than disulphide bonds.